高等学校化学学报

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (4): 646.

• 研究论文 • 上一篇    下一篇

3-磷酸甘油醛脱氢酶与酵母线粒体基因组维持蛋白Mgm101的相互作用

薛冬桦1,2, 王丽1, 左小明3   

  1. 1. 东北师范大学遗传与细胞研究所, 长春 130024;2. 长春工业大学生物工程学院, 长春 130012;
    3. 澳大利亚国立大学生命科学研究院酵母分子遗传实验室, 堪培拉 ACT 2601
  • 收稿日期:2005-09-06 出版日期:2006-04-10 发布日期:2006-04-10
  • 通讯作者: 王丽(1957年出生), 女, 教授, 博士生导师, 从事分子生物学研究. E-mail: wangli@nenu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 30471602)和国家留学基金(批准号： 20023034)资助.

Interaction of  Glyceraldehyde-3-phosphate Dehydrogenase in S.cerevisiae with Mitochndrial Genome
Maintenance Protein Mgm101

XUE Dong-Hua 1,2, WANG Li 1* ,  ZUO Xiao-Ming3   

  1. 1. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;
     2. School of Biological Engineering,Changchun University of Technology, Changchun 130012, China;
    3. Molecular Genetics and Evolution Group, Research School of Biological Science,
    the Australian National University, Canberra ACT 2601, Australia
  • Received:2005-09-06 Online:2006-04-10 Published:2006-04-10
  • Contact: WANG Li, wangli@nenu.edu.cn

PDF (PC)

被引次数

2

摘要：

采用酵母双杂交方法, 以Mgm101p为诱饵, 筛选酵母cDNA文库. 分离鉴定15个与Mgm101p相互作用的蛋白因子, 其中5个阳性克隆均为GPD1编码的3-磷酸甘油醛脱氢酶(GAPDH). 克隆了GPD1在 S. cerevisiae的同系物ScTDH2基因, 进行绿色荧光蛋白GFP标记、 亚细胞组分分离和蛋白质印迹分析，  结果表明， GAPDH除了在细胞质为糖酵解酶的主要作用外, 可能为多功能蛋白, 在酵母线粒体中与Mgm101p相互作用参与线粒体DNA维持的生物过程.

关键词: 3-磷酸甘油醛脱氢酶；  酵母线粒体DNA；  酵母双杂交；  Mgm101蛋白

Abstract:

MGM101 is involved in mitochondrial genome maintenance in yeast. Mgm101p is a protein required for the replication of mtDNA. By means of yeast two-hybrid, we screened a Schizosaccharomyces pombe cDNA library for clones encoding proteins that potentially interact with Mgm101p. We have isolated 15 mt-DNA-associated proteins in yeast. We had identified five individual clones with GPD1 coding for glyceraldehyde 3-phosphate dehydrogenase(GAPDH). The S. cerevisiae counterpart of the GPD1, TDH2 was cloned and examined for its possibility to be involved in mitochondrial genome maintenance. GFP tagging, subcellular fractionation followed by Western blotting analysis revealed that GAPDH, besides having a cytoplasmic location, was imported into mitochondria and had potential to interact with Mgm101p. These results suggest that GAPDH is likely a multifunctional protein and interacts with Mgm101p  in mitochondria, which is involved in the process mediated by mtDNA.

Key words: Glyceraldehyde-3-phosphate dehydrogenase; mtDNA; Yeast two-hybrid; Mgm101 protein

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