高等学校化学学报

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (4): 635.

• 研究快报 • 上一篇    下一篇

人卵巢癌耐药细胞株的比较蛋白质组分析

吕磊1,2,3, 刘志强1, 李丽1, 刘宁1, 刘淑莹1   

  1. 1. 中国科学院长春应用化学研究所新药研究实验室, 长春质谱中心, 长春 130022;
    2. 延安大学化学系, 延安 716000;  3. 中国科学院研究生院, 北京 100039
  • 收稿日期:2005-11-24 出版日期:2006-04-10 发布日期:2006-04-10
  • 通讯作者: 刘志强(1962年出生), 男, 博士, 研究员, 博士生导师, 从事天然药物化学和有机质谱学研究.E-mail: liuzq@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号: 20273067, 20173057)和中国科学院知识创新工程(批准号: KGCX2-SW-213-06)资助.

Comparative  Analysis of Proteomes for Chemoresistance in Human Ovarian Adenocarcinoma Cell Line,  COC1/DDP

LU Lei 1,2,3, LIU  Zhi-Qiang 1* , LI Li 1 , LIU Ning1, LIU Shu-Ying1   

  1. 1. Laboratory of  New Drug Research and Mass Spectrometry, Changchun Institute of Applied  Chemistry, Changchun Center of Mass Spectrometry, Changchun 130022,  China; 2. Department of Chemistry,
    Yan′an University, Yan′an 716000, China; 3. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
  • Received:2005-11-24 Online:2006-04-10 Published:2006-04-10
  • Contact: LIU Zhi-Qiang,E-mail: liuzq@ciac.jl.cn

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被引次数

12

摘要：

本文采用高分辨二维凝胶电泳分离技术对人卵巢癌细胞株COC1及其耐药细胞株COC1/DDP中的蛋白质进行分离和差异表达分析, 应用基质辅助激光解吸电离-飞行时间质谱对酶解多肽进行测定[即测定蛋白质的肽质量指纹图(Peptide mass fingerprinting, PMF)], 并通过相应的数据库搜索来鉴定蛋白质. 为获得更准确的检索结果, 采用串联质谱技术对各肽段进行氨基酸测序, 并应用IPI-HUMAN数据库对上述检索结果进一步加以确认. 

关键词: 卵巢癌; 蛋白质组; 质谱

Abstract:

The proteomic maps of  human ovarian adenocarcinoma cell line  in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE. The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment. Approximate 1300 protein spots were resolved in the two ranges of pI(3—10). The spots which have 10-fold difference in light density were digested by in-gel enzyme, identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) and finally detected by Profound and Mascot search engine in IDI databases. Seven proteins with obviously altered abundances were detected specifically, including four up-regulated protein spots and three down-regulated protein spots. The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the  therapy and pathogenesis.

Key words: Ovarian adenocarcinoma; Proteome; Mass spectrum

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