高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (3): 559.

• 研究简报 • 上一篇    下一篇

三种钌(Ⅱ)配合物与酵母RNA的键合动力学性质研究

蒋尚达,李世媛,崔孟超,刘霞,王科志   

  1. 北京师范大学化学系,北京 100875
  • 收稿日期:2005-02-26 出版日期:2006-03-10 发布日期:2006-03-10
  • 通讯作者: 王科志(1962年出生),男,博士,教授,博士生导师,从事功能配合物研究. E-mail: kzwang@bnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20371008和90401007)和北京师范大学本科生科学研究基金资助.

Binding Kinetics Properties of Three Ru(Ⅱ) Complexes with Yeast-RNA

JIANG Shang-Da,LI Shi-Yuan,CUI Meng-Chao,LIU Xia,WANG Ke-Zhi*   

  1. Department of Chemistry,Beijing Normal University,Beijing 100875,China
  • Received:2005-02-26 Online:2006-03-10 Published:2006-03-10

摘要:

本文采用紫外-可见光谱法研究了3个Ru(Ⅱ)金属配合物与酵母RNA的键合动力学.

关键词: 钌配合物;酵母RNA;动力学

Abstract:

The binding kinetics properties of three Ru(Ⅱ) complexes of [Ru(bpy)2(dhipH3)](ClO4)2,[(bpy)2Ru(eipcH)](ClO4)2 and [(bpy)2Ru(ebipcH2)Ru(bpy)2](ClO4)4,where bpy=2,2′-bipyridine,dhipH3=3,4-dihydroxyphenyl-(imidazo[4,5-f][1,10]phenanthroline),eipcH=N-ethyl-4-(imidazolo[4,5-f]-(1,10-phenanthrolin)-2-yl)carbazole,and ebipcH2=N-ethyl-4,7-bis(imidazolo[4,5-f]-(1,10-phenanthrolin)-2-yl)carbazole,with  yeast-RNA were investigated by monitoring the decays in absorbance for π-π* absorption peaks at 286 nm with binding time. The decay curves for the complexes were well fitting to biexponential decay functions with half-life times for rapidly-decayed components being 0.006,0.02 and 0.8 min,and those for slowly-decayed components being 0.7,4.2 and 103 min,respectively. The rapidly- and slowly-decayed components are probably due to electrostatic interaction and aromatic base stacking of the RNA with the Ru(Ⅱ) complexes,respectively. The results indicate that not only binuclear complexes but also mononuclear complexes can slowly bind to the RNA depending on the molecular structures of the complexes.

Key words: Ruthenium complex; Yeast RNA; Kinetics

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