Ag+生物吸附的谱学研究

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (3): 553.

Ag⁺生物吸附的谱学研究

薛茹¹，林种玉¹，郑建红²，王琪¹，颜长明¹，陈翠雪³，叶青¹，傅锦坤¹

1.厦门大学化学系固体表面物理化学国家重点实验室，2.厦门大学医院，
3.厦门大学化学工程与生物工程系,厦门361005

收稿日期:2005-03-15 出版日期:2006-03-10 发布日期:2006-03-10
通讯作者: 林种玉(1950年出生)，女，高级工程师，主要从事催化、生化等及有关谱学方面的研究. E-mail:stzylin@xmu.edu.cn

Spectroscopic Studies on Biosorption of Ag⁺

XUE Ru¹,LIN Zhong-Yu^1*,ZHENG Jian-Gong¹, WANG Qi²,YAN Chang-Ming¹,CHEN Cui-Xue³,YE Qing¹,FU Jin-Kun¹

1.State Key Laboratory of Physical Chemistry of Solid Surfaces,Department of Chemistry,Xiamen University;
2.Hospital of Xiaman University,
3.Department of Chemical Engineering,Xiamen University,Xiamen 361005,China

Received:2005-03-15 Online:2006-03-10 Published:2006-03-10

摘要/Abstract

摘要：

我们曾经研究了地衣芽孢杆菌R08和啤酒酵母废菌体吸附Pd²⁺以及巨大芽孢杆菌D01吸附Au³⁺过程的作用机理。有关乳酸杆菌A09吸附Ag⁺¹的作用特点已有报道。本文在此基础上，进一步用谱学技术研究A09菌体吸附还原Ag⁺的作用机理。

关键词: 生物吸附；Ag⁺；乳酸杆菌；生物还原；FTIR

Abstract:

The biosorptive interaction of Ag⁺ with resting cell of Lactobacillus sp. strain A09 has been further studied on a molecular level by means of XPS,EDX,UV-Vis and FTIR techniques. The X-ray photoelectron spectroscopy(XPS) shows that the reductive ratio of the Ag⁺ to Ag⁰ by the A09 biomass reaches to about 54.5% for 3 d. The contain of amino acid residues in dry powder of the biomass such as cysteine,methionine,arginine and lysine,being capable of reducing the Ag⁺ to Ag⁰,are very small in quantity both cysteine and methionine are far less than 0.18%,and both arginine and lysine far less than 0.336%,via the analysis with quantitative energy-dispersive X-ray(EDX). The amount of the reducing sugars in the biomass is far larger than 2.71% analyzed by ultraviolet-visible spectrophotometry(UV-Vis). The chemical functional group on cell wass of the biomass such as the carboxylate anion of amino-acid residues seems to be the site for the Ag⁺binding and the free aldehyde group of the hemiacetalic hydroxyl from reducing sugars,i.e. the hydrolysates of the polysaccharides from the cell wass,plays a protagonist in serving as the electron donor for reducing the Ag⁺to Ag⁰,characterized by fourier transform infrared(FTIR) spectrophotometry.

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TrendMD:

薛茹林种玉郑建红王琪颜长明陈翠雪叶青傅锦坤. Ag⁺生物吸附的谱学研究. 高等学校化学学报, 2006, 27(3): 553.

XUE Ru, LIN Zhong-Yu, ZHENG Jian-Gong, WANG Qi, YAN Chang-Ming, CHEN Cui-Xue, XIE Jing, FU Jin-Kun. Spectroscopic Studies on Biosorption of Ag⁺. Chem. J. Chinese Universities, 2006, 27(3): 553.