QDs标记免疫调节肽及其与T细胞作用的表征

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (2): 271.

QDs标记免疫调节肽及其与T细胞作用的表征

王丽凤¹, 王丽萍¹, 陈洁¹, 房学迅², 李惟¹

1. 吉林大学生命科学学院; 2. 吉林大学药学院, 长春 130023

收稿日期:2005-03-02 出版日期:2006-02-10 发布日期:2006-02-10
通讯作者: 李惟(1939年出生),男,教授,博士生导师,主要从事药物筛选与设计研究.E-mail:liwei@mail.jlu.edu.cn
基金资助:
长春市振兴老工业基地科技攻关课题(批准号: 04-02GG221)资助.

Quantum Dots Labeled Immune Peptide and Characterization of the Interaction with T Cell

WANG Li-Feng¹, WANG Li-Ping¹, CHEN Jie¹, FANG Xue-Xun², LI Wei¹

1. College of Life Science; 2. College of Pharmacy, Jilin University, Changchun 130023, China

Received:2005-03-02 Online:2006-02-10 Published:2006-02-10
Contact: LI Wei,E-mail:liwei@mail.jlu.edu.cn

摘要/Abstract

摘要：

量子点是直径为1~10 nm的球形半导体纳米晶体, 也被称为半导体量子点, 简称QDs. 与有机荧光染料相比, QDs具有激发光谱单一、荧光谱线窄、发光效率高、发光颜色可调、可进行多色联合标记, 并且光稳定性好等优点, 所以量子点是非常有前途的生物标记物[^1,2]. 研究结果表明, 量子点可以与许多生物分子如蛋白质、多肽、核酸及小分子配体等偶联. 现已有许多关于量子点标记生物分子的报道, 如用量子点标记木瓜蛋白酶、胰蛋白酶、天花粉蛋白和表皮生长因子等^[3-5].用量子点标记生物分子作为荧光探针已成功地应用于多种生物分析, 如DNA杂交监测、免疫分析和用QDs检测ATP推动的反应等^[4,6,7]. 目前, 对量子点标记生物分子的报道多为对大分子蛋白质的标记, 而对小分子肽标记的报道却很少.

关键词: 关键词半导体量子点(QDs); 荧光标记; 免疫调节肽

Abstract:

Abstract Semiconductor quantum dots(QDs) were used for labeling many biomacromolecules and small molecules, but it remains a challenge to couple it with short active peptides without any limitation, which play critical roles in many physiological processes. Several coupling methods known about QDs and short peptides have some limitations. In this research, we report a method for the synthesis of QDs labeled peptides to be appropriate to any short peptide. The QDs(CdTe)-labeled short peptides were verified and characterized by RP-HPLC. The result shows that the surface of the T cell treated with QDs-TP5 emits yellow fluoresence. These results indicate that QDs-TP5 tends to aggregate on the surface of T cells. They were applied to monitoring the specific binding between the immune peptides and T cell surface receptors. The binding and the resultant fluorescence were observed and monitored by fluorescence microscope in vitro. The QDs-labeled immune peptides provide a powerful method for studying the immune modulating activity of TP5 in vivo.

Key words: KeywordsSemiconductor quantum dots(QDs); Fluorescent labeling; Immune peptide

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王丽凤王丽萍陈洁房学迅李惟. QDs标记免疫调节肽及其与T细胞作用的表征. 高等学校化学学报, 2006, 27(2): 271.

WANG Li-Feng, WANG Li-Ping, CHEN Jie, FANG Xue-Xun, LI Wei. Quantum Dots Labeled Immune Peptide and Characterization of the Interaction with T Cell. Chem. J. Chinese Universities, 2006, 27(2): 271.