分子信标研究5-氟尿嘧啶对鼻咽癌HNE1细胞p33ING1基因转录水平的影响

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (2): 268.

分子信标研究5-氟尿嘧啶对鼻咽癌HNE1细胞p33^ING1基因转录水平的影响

王炜, 刘斌, 李军, 王柯敏, 谭蔚泓, 唐红星

湖南大学化学生物传感与计量学国家重点实验室, 湖南省生物纳米技术研究中心,
湖南大学生物医学工程中心, 长沙 410082

收稿日期:2005-06-22 出版日期:2006-02-10 发布日期:2006-02-10
通讯作者: 王柯敏(1957年生),男,博士,教授,博士生导师,从事纳米及单分子水平上的生物分析化学及纳米生物技术研究. E-mail: kmwang@hnu.cn
基金资助:
国家重点基础研究发展规划(批准号: 2002CB513110)、国家自然科学基金重点项目(批准号: 20135010)、国家自然科学基金青年基金(批准号: 20305006)及湖南省科研计划重点项目(批准号: 0399Y1006)资助

Investigation of the Effect of 5-FU on p33^ING1 mRNA Level Based on Molecular Beacon

WANG Wei, LIU Bin, LI Jun, WANG Ke-Min*, TAN Wei-Hong, TANG Hong-Xing

State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Engineering Research Center for
Bio-Nanotechnology, Biomedical Engineering Center, Hunan University, Changsha 410082, China

Received:2005-06-22 Online:2006-02-10 Published:2006-02-10
Contact: WANG Ke-Min，E-mail: kmwang@hnu.cn

摘要/Abstract

摘要：

p33^ING1作为一种重要的抑癌基因，在乳腺癌及胃癌等恶性肿瘤细胞中的mRNA表达水平显著低于癌旁正常组织细胞中的表达，并导致这些肿瘤细胞的侵袭转移能力增强，对化疗药物及r射线和紫外线处理的敏感性降低。

关键词: 分子信标; p33^ING1mRNA; 5-FU; 鼻咽癌; 药物敏感性

Abstract:

Abstract In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells, p33^ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay. During the operation, the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33^ING1 were measured in vitro. The results were as follows: p33^ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time. The MTT results also proved that high expression of p33^ING1mRNA can increase cell's sensitivity to chemical drug 5-FU. The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.

Key words: KeywordsMolecalar beacon; p33^ING1mRNA; 5-FU; Nasopharyngeal carcinoma; Drug sensitivity

中图分类号:

O629.74

TrendMD:

王炜刘斌李军王柯敏谭蔚泓唐红星. 分子信标研究5-氟尿嘧啶对鼻咽癌HNE1细胞p33^ING1基因转录水平的影响. 高等学校化学学报, 2006, 27(2): 268.

WANG Wei, LIU Bin, LI Jun, WANG Ke-Min, TAN Wei-Hong, TANG Hong-Xing. Investigation of the Effect of 5-FU on p33^ING1 mRNA Level Based on Molecular Beacon. Chem. J. Chinese Universities, 2006, 27(2): 268.