交联聚丙烯酰胺毛细管电泳涂层柱的制备及其应用

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (2): 218.

交联聚丙烯酰胺毛细管电泳涂层柱的制备及其应用

田宏哲, 杨丙成, 关亚风

中国科学院大连化学物理研究所, 现代分析与微型仪器研究室, 大连 116023

收稿日期:2005-05-23 出版日期:2006-02-10 发布日期:2006-02-10
通讯作者: 关亚风(1957年出生), 男, 博士，研究员, 博士生导师, 主要从事微型色谱研究. E-mail: kfguan@mail.dlptt.ln.cn
基金资助:
国家自然科学基金(批准号: 20227501)资助

Preparation and Application of Cross-linked Polyacrylamide Coated Column for Capillary Electrophoresis

TIAN Hong-Zhe, Yang Bing-Cheng, Guan Ya-Feng

Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of
Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Received:2005-05-23 Online:2006-02-10 Published:2006-02-10
Contact: Guan Ya-Feng，E-mail: kfguan@mail.dlptt.ln.cn

摘要/Abstract

摘要：

摘要建立了一种简便的制备交联聚丙烯酰胺型毛细管电泳涂层柱的方法. 所制备的涂层柱能够有效地抑制电渗流及蛋白质在管壁上的吸附. 考察了碱性蛋白质在pH=4。0的缓冲溶液中的分离, 迁移时间重现性误差小于2。1%. 对麻黄提取物中的生物碱进行了分离, 平均理论塔板数为24×105 plates/m.

关键词: 关键词交联聚丙烯酰胺涂层柱; 毛细管电泳; 蛋白分离; 生物碱分离

Abstract:

Abstract A method was developed for the preparation of static coating onto the fused-silica columns with cross-linked polyacrylamide. The coated columns were effective to suppress the electroosmotic flow and prevent the absorption of basic proteins on the column wall. The basic proteins were separated by using the coated columns at pH=4.0, with retention time variation of less than 2.1% RSD. The average separation efficiencies were 2.4×105 plates/m for alkaloids. The cross-linked polyacrylamide coated columns can be prepared reproducibly by using this method.

Key words: KeywordsCross-liked polyacrylamide-coated column; Capillary electrophoresis; Protein separation; Alkaloid separation

中图分类号:

0658

TrendMD:

田宏哲杨丙成关亚风. 交联聚丙烯酰胺毛细管电泳涂层柱的制备及其应用. 高等学校化学学报, 2006, 27(2): 218.

TIAN Hong-Zhe, Yang Bing-Cheng, Guan Ya-Feng. Preparation and Application of Cross-linked Polyacrylamide Coated Column for Capillary Electrophoresis. Chem. J. Chinese Universities, 2006, 27(2): 218.