高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (1): 62-66.

• 研究论文 • 上一篇    下一篇

解偶联剂CCCP对莱茵衣藻光照产氢过程的调控

冉春秋1, 2, 张卫1, 虞星炬1,  金美芳1, 邓麦村1   

  1. 1. 中国科学院大连化学物理研究所海洋生物产品工程组, 大连116023;
    2. 中国科学院研究生院, 北京 100039
  • 收稿日期:2004-12-27 出版日期:2006-01-10 发布日期:2006-01-10
  • 通讯作者: 张卫(1969年出生), 男, 博士, 研究员, 博士生导师, 从事海洋绿藻可逆产氢酶生物光水解制氢的理论和技术研究. E-mail: weizhang@dicp.ac.cn
  • 基金资助:

     国家科技部“九七三”计划项目(批准号: 2003CB214506)资助.

Regulation of Hydrogen Production by Uncoupler CCCP in Green Algae Chlamydomonas reinhardtii

RAN Chun-Qiu 1, 2,  ZHANG Wei 1* ,  YU Xing-Ju1,  JIN Mei-Fang1,  DENG Mai-Cun1   

  1. 1. Marine Bioproducts Engineering Group,  Dalian Institute of Chemical Physics,Chinese Academy of Sciences,  Dalian 116023,  China;
    2 Graduate School of Chinese Academy of Sciences,  Beijing  100039, China
  • Received:2004-12-27 Online:2006-01-10 Published:2006-01-10
  • Contact: ZHANG Wei, E-mail: weizhang@dicp.ac.cn

摘要:

本文研究莱茵衣藻在含硫和缺硫连续光照条件下, 不同浓度的CCCP对PSⅡ光化学效率、 光照产氢、 光合放氧以及光能的吸收和转换效率等的影响和调控规律.

关键词: 羰基氰化物间氯苯腙(CCCP); 莱茵衣藻; 光化学活性; 氢气; 氢酶

Abstract:

 Uncoupler,  carbonylcyanide-m-chlorophenylhydrazone[CCCP],  can markedly inhibit the photochemical activity of photosystem Ⅱ and repress the rate of photosynthetic oxygen evolution.  As a result,  the anaerobic condition can promptly induce hydrogenase expression and accelerate hydrogen photoproduction under the  continuous illumination by Chlamydomonas reinhardtii.  When C. reinhardtii cultured in TAP medium and treated with 0,  5 and   15 μmol/L CCCP under the continuous illumination,  the photochemical activity of algae could not be obviously inhibited.  But 15 and 20 μmol/L CCCP could markedly depress the photochemical activity and substantial amount of hydrogen gas was photoproduced.  The photochemical activity of C. reinhardtii cultured with TAP-S at all concentrations of CCCP under the continuous llumination was distinctly inhibited and the cultures photoproduced hydrogen gas rapidly.  The algae of C. reinhardtii cultured with TAP and TAP-S medium,  different concentrations of CCCP altered the process of hydrogen metabolism and the efficiency of utilization and conversion of solar energy by the center of PSⅡ.

Key words: Carbonylcyanide\, m\, chlorophenylhydrazone(CCCP); Chlamydomonas reinhardtii; Photochemical activity; Hydrogen gas; Hydrogenase

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