高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (6): 1090.

• 研究简报 • 上一篇    下一篇

猪精浆中附睾特异性蛋白质的纯化及其一级结构的研究

邱芳萍1, 杜培革2, 安丽萍2, 吕刚2, 李青山3   

  1. 1. 长春工业大学生物工程学院, 长春130012;
    2. 北华大学医学院, 吉林132000;
    3. 吉林大学生命科学学院, 长春130021
  • 收稿日期:2004-08-05 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 邱芳萍(1955年出生),女,教授,从事生物化学与分子生物学研究.E-mail:qfp2004@126.com E-mail:qfp2004@126.com
  • 基金资助:

    吉林省科学技术厅基础研究项目(批准号:2004128)资助.

Purification of Epididymis-specific Protein from Porcine Seminal Plasmas and Its Primary Structure

QIU Fang-Ping1, DU Pei-Ge2, AN Li-Ping2, LV Gang2, LI Qing-Shan3   

  1. 1. College of Biological Engineering, Changchun University of Technology, Changchun 130012, China;
    2. Medical College, Beihua University, Jilin 132000, China;
    3. College of Life Sience, Jilin University, Changchun 130023, China
  • Received:2004-08-05 Online:2005-06-10 Published:2005-06-10

PDF (PC)

被引次数

1

关键词: 猪, 附睾, 特异性蛋白, 提纯, 一级结构

Abstract: Through successive chromatography on Q-sepharose, Matrex Red A, Superdex S-200 and Resource Q chromatography column, epididymis-specific protein from porcine seminal plasma was purified. Epididymis-specific protein(PE4) was purified to homogeneity by polyacrylamide gel electrophoresis. The molecular weight of the purified protein was calculated to be about 11 000 on SDS-PAGE. The whole primary structure of the purified epididymis-specific protein was determined by N-terminal amino acid sequence analysis, digestion of lysyl-endo-peptidase and BrCN. The primary structure of PE4 showed a significant similarity to those of epididymis-specific protein-4(HE4). The level of amino acid homology was 78.49%. Then, it indicates that epididymis-specific protein belonged to disulfide-core-protein of antileukocyte proteinase inhibitor(ALP) and there is a important function in sperm maturation.

Key words: Porcine, Epididymis, Specific protein, Purification, Primary structure

中图分类号: 

TrendMD: