高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (6): 1046.

• 研究快报 • 上一篇    下一篇

可溶性耐药相关钙结合蛋白的生物质谱分析与鉴定

刘宁1,2, 齐静3, 吕磊1, 刘志强1, 刘淑莹1, 杨纯正2   

  1. 1. 中国科学院长春应用化学研究所新药研究实验室, 长春130022;
    2. 吉林大学生命科学技术研究所, 长春130021;
    3. 中国医学科学院血液学研究所实验血液学国家重点实验室, 天津300020
  • 收稿日期:2005-01-06 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 刘志强(1962年出生),男,博士,研究员,博士生导师,主要从事质谱分析研究.E-mail:liuzq@ciac.jl.cn E-mail:liuzq@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号:20273067)资助.

Identification of Soluble Resistance-related Calcium Binding Protein by Bio-mass Spectrometric Analysis

LIU Ning1,2, QI Jing3, LV Lei1, LIU Zhi-Qiang1, LIU Shu-Ying1, YANG Chun-Zheng2   

  1. 1. Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. Institute of Life Science and Technology, Jilin University, Changchun 130021, China;
    3. State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300020, China
  • Received:2005-01-06 Online:2005-06-10 Published:2005-06-10

PDF (PC)

被引次数

4

关键词: 可溶性耐药相关钙结合蛋白, 基质辅助激光解吸离子化飞行时间质谱, 二维凝胶电泳

Abstract: The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4.7-59. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein(sorcin). Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.

Key words: Soluble resistance-related calcium binding protein, Matrix-assisted laser desorption ionization time of flight mass spectrometry, Two-dimensional gel electrophoresis

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