高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (5): 829.

• 研究快报 • 上一篇    下一篇

纳米电极-膜片钳监测单个活囊泡连续释放

吴文展1, 黄卫华1, 王玮1, 王宗礼1, 程介克1, 张蓉颖2, 陈宇2, 刘杰2, 郑从义3, 沈超3   

  1. 1. 武汉大学化学与分子科学学院, 武汉430072;
    2. 华中科技大学生命科学与技术学院, 武汉430074;
    3. 武汉大学生命科学院, 武汉430072
  • 收稿日期:2005-02-03 出版日期:2005-05-10 发布日期:2005-05-10
  • 通讯作者: 程介克(1930年出生),男,教授,博士生导师,主要从事微流控芯片、单细胞单分子检测研究.E-mail:jkcheng@whu.edu.cn; 郑从义(1953年出生),男,教授,博士生导师,从事细胞生物学研究.E-mail:cctcc@whu.edu.cn E-mail:jkcheng@whu.edu.cn; cctcc@whu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20299034;20405012)资助.

Nanoelectrode-patch Clamp Monitoring of Continual Single Living Vesicle Release

WU Wen-Zhan1, HUANG Wei-Hua1, WANG Wei1, WANG Zong-Li1, CHENG Jie-Ke1, ZHANG Rong-Ying2, CHEN Yu2, LIU Jie2, ZHENG Cong-Yi3, SHEN Chao3   

  1. 1. College of Chemistry & Molecular Science, Wuhan University, Wuhan 430072, China;
    2. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China;
    3. College of Life Science, Wuhan University, Wuhan 430072, China
  • Received:2005-02-03 Online:2005-05-10 Published:2005-05-10

PDF (PC)

被引次数

4

关键词: 纳米电极, 膜片钳, 单囊泡空间分辨, 连续囊泡释放

Abstract: Carbon fiber nanoelectrode(tip diameter ca. 100 nm)-patch clamp was firstly applied to real-time monitoring dopamine release from single living vesicles of single Rat pheochromocytoma(PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis.

Key words: Nanoelectrode, Patch clamp, Single vesicle spatial resolution, Continual vesicle release

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