高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (4): 693.

• 研究论文 • 上一篇    下一篇

丹酰基丝氨酸与桥联双环糊精作用机理的时间分辨荧光光谱研究

陈凯1, 贾树岩1, 郝雅琼1, 吴立新1, 吴玉清1, 刘俊秋1, 刘瑞斌2, 张建平2, 艾希成2   

  1. 1. 吉林大学超分子结构和材料教育部重点实验室, 长春 130012;
    2. 分子动态和稳态结构国家重点实验室, 中国科学院化学研究所分子科学中心, 北京 100080
  • 收稿日期:2004-09-14 出版日期:2005-04-10 发布日期:2005-04-10
  • 通讯作者: 吴玉清(1966年出生),女,博士,教授,从事生物分子识别的光谱学研究.E-mail:yqwu@jlu.edu.cn E-mail:yqwu@jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20373017,20473028和20003004);国家重点基础研究发展规划(批准号:G2000078102)资助.

Time-resolved Fluorescence Spectroscopic Studies on Interaction Mechanism of Dansyl-L-serine with Bridged-bicyclodextrins

CHEN Kai1, JIA Shu-Yan1, HAO Ya-Qiong1, WU Li-Xin1, WU Yu-Qing1, LIU Jun-Qiu1, LIU Rui-Bin2, ZHANG Jian-Ping2, AI Xi-Cheng2   

  1. 1. Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, China;
    2. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center of Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
  • Received:2004-09-14 Online:2005-04-10 Published:2005-04-10

摘要: 利用稳态、时间分辨荧光光谱,结合非线性最小二乘法拟合系统研究了手性探针分子丹酰-L-丝氨酸(DLS)与β-环糊精(β-CD)、2-位硒桥联β-CD(1)和2-位碲桥联β-CD(2)的复合及复合机理.对比研究了DLS与三种不同环糊精复合强度及复合方式的差别.结果表明,引入硒桥键不能使化合物1与丹酰基丝氨酸分子很好地复合;而引入碲桥键使化合物2对DLS分子的结合能力相对于β-CD有明显增强.

关键词: 丹酰基丝氨酸, 硒桥联双环糊精, 碲桥联双环糊精, 非线性最小二乘法拟合, 时间分辨荧光光谱

Abstract: Time-resolved fluorescence spectra were applied to study the binding of dansyl-L-serine(DLS) with β-cyclodextrin(β-CD), 2-selenium bridged β-cyclodextrin(1) and 2-tellurium bridged β-cyclodextrin(2). The differentiations of DLS in binding with three kinds of cyclodextrins were performed. The results suggest that compound 1 cannot bind with DLS efficiently. However, compound 2 can enhance the binding ability with DLS greatly in comparison with that of β-CD.

Key words: Dansyl-L-serine, 2-Selenium bridged β-cyclodextrin, 2-Tellurium bridged β-cyclodextrin, Non-linear least-square curve fitting, Time-resolved fluorescence spectroscopy

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