高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (12): 2259.

• 研究论文 • 上一篇    下一篇

HIV-1嵌合抗原的纯化及免疫原性分析

蒋静1, 赵丽辉1,2,于湘晖1,姜春来1,吴永革1,貌盼勇3,李惟1,孔维1   

  1. 1.吉林大学生命科学学院,长春130012;2.吉林农业大学园艺学院,长春130118;
    3.解放军302医院病毒研究室,北京100039
  • 收稿日期:2005-03-25 出版日期:1905-03-14 发布日期:1905-03-14
  • 通讯作者: 孔维（1965年出生），男，博士，教授，博士生导师，从事生物化学和分子生物学研究.E-mail：weikong@jlu.edu.cn
  • 基金资助:

    国家自然科学基金（批准号：3017025）资助.

Purification and Analysis of Antigencity of Chimeric Antigen of HIV-1

JIANG Jing1, ZHAO Li-Hui1, 2, YU Xiang-Hui1, JIANG Chun-Lai1, WU Yong-Ge1, MAO Pan-Yong3, LI Wei1, KONG Wei1*   

  1. 1. College of Life Science, JiLin University, Changchun 130012, China; 2. College of Agriculture, Jilin Agriculture
    University, Changchun 130118, China; 3. Department of Virology, 302 Hospital of PLA, Beijing 100039, China
  • Received:2005-03-25 Online:1905-03-14 Published:1905-03-14
  • Contact: KONG Wei;E-mail：weikong@jlu.edu.cn

被引次数

4

摘要：

为获得高效的HIV诊断试剂,选定HIV-1的外膜蛋白env中469-511aa,538-674aa和700-734aa3处包含较多抗原位点的区域作为免疫抗原,用PCR的方法从HIV-1全基因扩增编码这3处片段的基因序列,将它们克隆到原核表达载体中,利用大肠杆菌表达系统表达嵌合蛋白.结果发现,3段嵌合基因能在大肠杆菌BL21(Star)中表达,通过Ni-sepharose 4B金属Ni螯合层析柱分离纯化目的产物,酶联免疫检测结果表明,纯化抗原有较强的抗原特异性.

关键词: env基因;嵌合抗原;免疫原性;免疫学诊断, env基因, 嵌合抗原, 免疫原性, 免疫学诊断

Abstract:

In order to get the high-efficient diagnosing reagent of HIV, we choose three segments of HIV-1 envelope protein as the immune antigen, 469-511aa, 538-674aa and 700-734aa, which include more epitope. These three segment genes were obtained by PCR amplification and cloned into prokaryotic expressing vector. The results show that chimeric env protein can be expressed in E. coli BL21 strain high efficiently. We purified the protein by metal Ni-sepharose 4B, the data of ELISA demonstrate that purified chimeric antigen has good antigenic specificity.

Key words: env Gene; Chimeric antigen; Antigenicity; Immunology diagnosing, env Gene, Chimeric antigen, Antigenicity, Immunology diagnosing

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