高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (12): 2230.

• 研究简报 • 上一篇    下一篇

高效液相色谱-电喷雾串联四极杆质谱法研究人尿中毛果芸香碱代谢产物

王玲1,王英武2,陈刚1,袁宏明3   

  1. 1.吉林大学测试科学实验中心;2.生命科学学院药物代谢研究中心;3.化学学院,长春130023
  • 收稿日期:2005-05-20 出版日期:1905-03-14 发布日期:1905-03-14
  • 通讯作者: 袁宏明(1961年出生),男,博士,副教授,主要从事手性分子研究.E-mail:hmyuan@jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20471021)资助.

Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry

WANG Ling1,WANG Ying-Wu2,CHEN Gang1,YUAN Hong-Ming3*   

  1. 1.Analysis Test & Experiment Center;2.Research Center for Drug Metabolism,College of Life Science;  3.College of Chemistry,Jilin University,Changchun 130023,China
  • Received:2005-05-20 Online:1905-03-14 Published:1905-03-14
  • Contact: YUAN Hong-Ming;E-mail:hmyuan@jlu.edu.cn

摘要:

本文采用高效液相色谱-电喷雾串联四极杆质谱法对人口服毛果芸香碱后的尿样的代谢产物进行了研究.

关键词: 毛果芸香碱;代谢物;液相色谱-串联质谱法, 毛果芸香碱, 代谢物, 液相色谱-串联质谱法

Abstract:

Metabolism of pilocarpine in human urine was studied by high-performance liquid chromatography with tandem mass spectrometric method after single oral dose 10 mg pilocarpine hydrochloride to a healthy male volunteer.The urine sample was collected from 0 to 8 h after oral administration.The urine sample was prepared by solid phase extraction,and then was analyzed  using high-performance liquid chromatography coupled with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring and product ion scan mode.Three metabolites were identified as the oxidative metabolite,pilocarpic acid and the glucuronide of pilocarpine.

Key words: Pilocarpine; Metabolite; Liquid chromatography-tandem mass spectrometry, Pilocarpine, Metabolite, Liquid chromatography-tandem mass spectrometry

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