高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (1): 49.

• 研究简报 • 上一篇    下一篇

CaCl2作为异硫氰酸曙红固体基质室温磷光增强剂及其在免疫分析中的应用

吴娅兰1, 李隆弟1, 刘佳铭2, 朱国辉2   

  1. 1. 清华大学化学系,北京 100084;
    2. 漳州师范学院化学系,漳州 363000
  • 收稿日期:2004-03-08 出版日期:2005-01-10 发布日期:2005-01-10
  • 通讯作者: 李隆弟(1939年出生),男,教授,从事光致发光和分子识别机理及应用研究.E-mail:lild@chem.tsinghua.edu.cn E-mail:lild@chem.tsinghua.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20175011);福建省自然科学基金(批准号:C0110028)资助

Solid Substrate Room Temperature Phosphorescence of Eosin-5-isothiocynate and Application in Immune Analysis Using CaCl2 as an Enhancer

WU Ya-Lan1, LI Long-Di1, LIU Jia-Ming2, ZHU Guo-Hui2   

  1. 1. Department of Chemistry, Tsinghua University, Beijing 100084, China;
    2. Department of Chemistry, Zhangzhou Normal Institute, Zhangzhou 363000, China
  • Received:2004-03-08 Online:2005-01-10 Published:2005-01-10

关键词: 固体基质室温磷光免疫分析法, 异硫氰酸曙红, CaCl2作增强剂, 人IgG

Abstract: Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λexem=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔIP was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression equation of working curve can be expressed as ΔIP=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3Sb/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.

Key words: Solid substrate room temperature phosphorescence immunoassay, Eosin-5-isothiocynate, CaCl2 as an enhancer, IgG

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