高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (S1): 165.

• 研究论文 • 上一篇    下一篇

Studies on Enzyme Kinetics by Microchip and Related Techniques

James J. Bao1, WANG Huai-Feng1, ZHOU Da-Wei1, Ken R. Wehmeyer2   

  1. 1. College of Pharmaceuticals, Tianjin University, Tianjin 300072, China;
    2. Procter & Gamble Pharmaceuticals, 8700 Mason-Montgomery Road, Mason, OH 45040, USA
  • 出版日期:2004-12-31 发布日期:2004-12-31

Studies on Enzyme Kinetics by Microchip and Related Techniques

James J. Bao1, WANG Huai-Feng1, ZHOU Da-Wei1, Ken R. Wehmeyer2   

  1. 1. College of Pharmaceuticals, Tianjin University, Tianjin 300072, China;
    2. Procter & Gamble Pharmaceuticals, 8700 Mason-Montgomery Road, Mason, OH 45040, USA
  • Online:2004-12-31 Published:2004-12-31

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摘要：

Both conventional and microchip-based capillary electrophoresis(CE) technologies have been used for the analysis of enzymes.Practical procedures of using CE to determine the Km and Vmax values of an enzyme have been developed.By studying the inhibition to the enzyme,it is possible to select a suitable drug candidate.When compared with the conventional CE method,single lane microchip-based method can improve the speed for the assay three times.By using multiple lane-based microchip,the speed can be further increased.

关键词: Enzyme, Enzyme kinetics, Inhibition, Enzyme activity

Abstract:

Both conventional and microchip-based capillary electrophoresis(CE) technologies have been used for the analysis of enzymes.Practical procedures of using CE to determine the Km and Vmax values of an enzyme have been developed.By studying the inhibition to the enzyme,it is possible to select a suitable drug candidate.When compared with the conventional CE method,single lane microchip-based method can improve the speed for the assay three times.By using multiple lane-based microchip,the speed can be further increased.

Key words: Enzyme, Enzyme kinetics, Inhibition, Enzyme activity

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