以石英晶体微天平-流动注射系统动态测定生物分子相互作用

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (S1): 159.

以石英晶体微天平-流动注射系统动态测定生物分子相互作用

张红武, 赵睿, 陈志勇, 上官棣华, 刘国诠

中国科学院化学研究所分子科学中心, 北京 100080

出版日期:2004-12-31 发布日期:2004-12-31
通讯作者: 赵睿
基金资助:
国家自然科学基金(批准号:20075033,20035010,20275040)和中国科学院基金(批准号:KJCX2-SW-H06)资助.

Dynamic Determination of Bio-molecular Interaction Using Quartz Crystal Microbalance-Flow Injection System

ZHANG Hong-Wu, ZHAO Rui, CHEN Zhi-Yong, SHANGGUAN Di-Hua, LIU Guo-Quan

Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

Online:2004-12-31 Published:2004-12-31

摘要/Abstract

摘要：

生命过程均涉及到生物分子间的相互作用.开展生物分子间相互识别的动力学研究,获知其相互作用过程的动态信息,将有助于了解其作用机理,并在分子水平上认识生物分子结构与功能之间的关系.

关键词: QCM-FIA, 动态相互作用, 肝素与抗凝血酶Ⅱ

Abstract:

The interaction between heparin and antithrombin Ⅱ was studied by means of the homemade QCM-FIA and the related calculation software.The heparin molecules were covalently immobilized onto the surface of gold electrode,on which a layer of poly(glycidyl methacrylate) thin membrane was constructed in advance,via the interaction of epoxy groups with amino groups of heparin molecules.The experimental results obtained accord with the data obtained from the related affinity chromatography experiment.

Key words: QCM-FIA, Dynamic Interactian, Heparin-antithrombin Ⅱ

TrendMD:

张红武, 赵睿, 陈志勇, 上官棣华, 刘国诠. 以石英晶体微天平-流动注射系统动态测定生物分子相互作用. 高等学校化学学报, 2004, 25(S1): 159.

ZHANG Hong-Wu, ZHAO Rui, CHEN Zhi-Yong, SHANGGUAN Di-Hua, LIU Guo-Quan. Dynamic Determination of Bio-molecular Interaction Using Quartz Crystal Microbalance-Flow Injection System. Chem. J. Chinese Universities, 2004, 25(S1): 159.