儿茶酚胺衍生物与DNA之间相互作用的光谱和电化学法研究

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (7): 1235.

儿茶酚胺衍生物与DNA之间相互作用的光谱和电化学法研究

杨功俊, 徐静娟, 陈洪渊

南京大学化学系分析科学研究所配位化学国家重点实验室, 南京 210093

收稿日期:2003-05-21 出版日期:2004-07-24 发布日期:2004-07-24
通讯作者: 陈洪渊(1937年出生),男,教授,博士生导师,中国科学院院士,主要从事生物电化学和生化分析研究.E-mail:hychen@netra.nju.edu.cn E-mail:hychen@netra.nju.edu.cn
基金资助:
国家自然科学基金(批准号:90206037,20205007,20075010)资助

Studies on the Interaction Between Catecholamine Derivatives and DNA by Means of Spectroscopic and Voltammetric Methods

YANG Gong-Jun, XU Jing-Juan, CHEN Hong-Yuan

Institute of Analytical Science, State Key Laboratory of Coordination Chemistry, Department of Chemistry, Nanjing University, Nanjing 210093, China

Received:2003-05-21 Online:2004-07-24 Published:2004-07-24

摘要/Abstract

摘要： 用光谱和电化学方法研究了儿茶酚胺衍生物与小牛胸腺DNA之间的作用机制.结果表明,低浓度的多巴酚丁胺和肾上腺素与DNA之间主要为静电作用;而在高浓度时,则主要为插入作用.对于多巴胺,在5.00×10^-5～9.00×10^-4mol/L范围内,它与DNA之间主要为插入作用.同时,采用电化学方法测得多巴胺、肾上腺素和多巴酚丁胺与DNA之间的表观结合常数分别为1.55×10³,9.77×10³和1.74×10⁴L/mol.

关键词: 小牛胸腺DNA, 溴化乙锭, 儿茶酚胺衍生物, 光谱和电化学方法

Abstract: The interactions between catecholamine derivatives and DNA were investigated by means of cyclic voltammetry, UV-Vis absorption spectra and fluorescence spectra. The results show that the interaction mode is mainly electrostatic interaction at the low concentration of dobutamine and adrenaline whereas intercalative binding plays a dominant role at their high concentration. As for dopamine, it intercalates into the double helix of DNA in the concentration range of 5.00×10^-5 to 9.00×10^-4 mol/L. The binding constants of dopamine, adrenaline and dobuamine with DNA are determined as 1.55×10³, 9.77×10³ and 1.74×10⁴ L/mol, respectively, by electrochemical method.

Key words: ctDNA, Ethidium bromide, Catecholamine derivatives, Spectroscopic and voltammetric methods

中图分类号:

O612.2

TrendMD:

杨功俊, 徐静娟, 陈洪渊. 儿茶酚胺衍生物与DNA之间相互作用的光谱和电化学法研究. 高等学校化学学报, 2004, 25(7): 1235.

YANG Gong-Jun, XU Jing-Juan, CHEN Hong-Yuan . Studies on the Interaction Between Catecholamine Derivatives and DNA by Means of Spectroscopic and Voltammetric Methods. Chem. J. Chinese Universities, 2004, 25(7): 1235.

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