高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (7): 1231.

• 研究论文 • 上一篇    下一篇

基于复合型微流控芯片的紫外检测毛细管电泳系统

曹小丹, 方群, 方肇伦   

  1. 浙江大学化学系微分析系统研究所, 杭州 310028
  • 收稿日期:2003-08-12 出版日期:2004-07-24 发布日期:2004-07-24
  • 通讯作者: 方 群(1966年出生),男,博士,教授,从事微流控分析芯片的研究.E-mail:fangqun@mail.hz.z.jcn E-mail:fangqun@mail.hz.z.jcn
  • 基金资助:

    国家自然科学基金重大项目(批准号:20299030);国家“八六三”计划项目(批准号:2002AA2Z2042);教育部科学技术研究重点项目(批准号:01093)资助

A Hybrid Microfluidic Chip-based Capillary Electrophoresis System with UV Absorbance Detection

CAO Xiao-Dan, FANG Qun, FANG Zhao-Lun   

  1. Institute of Microanalysis System, Department of Chemistry, Zhejiang University, Hangzhou 310028, China
  • Received:2003-08-12 Online:2004-07-24 Published:2004-07-24

摘要: 提出了一种基于芯片-毛细管复合装置的紫外检测-微流控芯片毛细管电泳分析系统.采用小死体积的耦合技术实现了石英毛细管与“十”字通道型微流控玻璃芯片的耦合.本系统的紫外检测灵敏度与商品化毛细管电泳仪相当.采用夹流进样方式,达到较高的进样重现性,2mmol/L苯甲酸的峰高相对标准偏差(RSD)为1.5%(n=11).可用于复方磺胺甲唑片剂的两种有效成分的快速分离.

关键词: 毛细管-芯片耦合系统, 微流控芯片毛细管电泳, 紫外检测

Abstract: A hybrid microfluidic system produced by coupling a microfabricated glass chip with conventional silica capillary for UV absorbance detection was developed. The system has proved to be robust, and easily fabricated at a low cost. The effects of sample loading and separation field strength on plate number, migration time and signal response were observed in the optimization studies. The reproducibility was examined by using benzoic acid as a model sample and following similar behavior as for complete photolithographically produced glass chip systems. The results showed that the reproducibility is good and the RSD(n=11) was only 1.5% for benzoic acid. The performance of the system was demonstrated by separating sulphamethoxazole(SMZ) and trimethoprim(TMP) in sulphatrim tablets within 70 s. The detection limits were comparable to those obtained with a commercial capillary electrophoresis instrument.

Key words: Microfluidic chip-silica capillary hybrid system, Microfluidic capillary electrophoresis, UV detection

中图分类号: 

TrendMD: