高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (7): 1226.

• 研究论文 • 上一篇    下一篇

蛋白质酶解混合物的动态修饰毛细管电泳-质谱联用分析

王晓燕1, 牟晓盾1, 许云敏2, 杨原1, 王洪海2   

  1. 1. 复旦大学化学系;
    2. 复旦大学遗传研究所, 上海 200433
  • 收稿日期:2003-09-04 出版日期:2004-07-24 发布日期:2004-07-24
  • 通讯作者: 杨 原(1949年出生),男,教授,博士生导师,从事生物质谱分析研究.E-mail:pyyang@fudan.edu.cn E-mail:pyyang@fudan.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20205002);国家重点研究专项基金(批准号:2001CB5102)资助

Analysis of Protein-digested Mixture with Dynamic Coating Capillary Electrophoresis-Mass Spectrometry

WANG Xiao-Yan1, MOU Xiao-Dun1, XU Yun-Min2, YANG Peng-Yuan1, WANG Hong-Hai 22   

  1. 1. Department of Chemistry;
    2. Institute of Genetics, Fudan University, Shanghai 200433, China
  • Received:2003-09-04 Online:2004-07-24 Published:2004-07-24

摘要: 利用羟丙基纤维素溶液动态涂层技术修饰毛细管管壁,改善了分离效率.在不影响质谱检测的条件下,将动态涂层毛细管电泳与质谱检测联用,有效地提高了对蛋白质的鉴定能力.将该技术应用于对复杂蛋白质样品的酶解产物的分析鉴定,结果令人满意.

关键词: 羟丙基纤维素, 动态涂层, 毛细管电泳-质谱联用(CE-MS), 蛋白质鉴定

Abstract: An improved method of mass compatible dynamic coating was developed for proteins identification with capillary electrophoresis-mass spectrometry(CE/MS), which kept the high separation efficiency of dynamic coating and eliminated the negative effects of coating reagent to MS detection. The identification efficiency of protein by the peptide mapping of tryptic digested protein was improved. The complex protein mixture was analyzed and successfully identified by this method. The identification efficiency of protein by the peptide mapping to tryptic digested protein was dramatically improved, that the MAscot score of myoglobin digest analysis on CE/MS was increased from304 to 914 with the dynamic coating. Four protein tryptic digests mixture was also analyzed and successfully identified by this method.

Key words: Hydropropyl cellulose, Dynamic coating, Capillary electrophoresis-mass spectrometry(CE-MS), Protein identification

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