GC-MS与正交投影法或渐进窗口正交投影法用于羌活挥发油成分的测定

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (3): 430.

GC-MS与正交投影法或渐进窗口正交投影法用于羌活挥发油成分的测定

郭方遒, 梁逸曾, 黄兰芳, 徐承建

中南大学化学化工学院中草药现代化研究中心, 长沙 410083

收稿日期:2003-04-29 出版日期:2004-03-24 发布日期:2004-03-24
通讯作者: 梁逸曾(1950年出生),男,教授,博士生导师,从事分析化学和中药现代化研究.E-mail;yzliang@public.cs.hn.cn E-mail:yzliang@public.cs.hn.cn
基金资助:
国家自然科学基金(批准号;2017503;20235020)资助

Determination of the Volatile Chemical Constituents of Notoptergium Incium by GC-MS and OPA or EWOP

GUO Fang-Qiu, LIANG Yi-Zeng, HUANG Lan-Fang, XU Cheng-Jian

Research Center for Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

Received:2003-04-29 Online:2004-03-24 Published:2004-03-24

摘要/Abstract

摘要： 中药化学组成的定性定量测定是药物活性理论的基础,联用色谱及其相关的化学计量学方法为中药复杂体系的分离和分辨提供了强有力的工具.采用GC-MS法对传统中药羌活中的挥发油成分进行了分离测定,并对其中重叠色谱峰根据其重叠程度,采用迭代的正交投影法(OPA)和非迭代的渐进窗口正交投影法(EWOP)进行了分辨,得到每个组分的纯色谱和光谱曲线,共分辨出98个色谱峰,通过质谱库检索得到其中65个组分的定性定量结果,占总含量的92.13%.

关键词: 气相色谱-质谱(GC-MS)联用, 渐进窗口正交投影法(EWOP), 正交投影法(OPA), 羌活, 挥发油

Abstract: The qualitative and quantitative determination of the chemical constituents in traditional Chinese medicines(TCMs) is an important task, which builds the foundation of the theory of pharmacological activity. The hyphenated chromatographic instruments combined with the related chemometric methods provide powerful tools for the resolution of such complex systems. The volatile chemical constituents of Notoptergium incium (NI) were analyzed by gas chromatography mass spectrometry(GC MS) and the overlapped peaks were resolved into pure chromatograms and pure spectra with iterative OPA or non iterative EWOP chemometrics resolution method. Sixty five of the 98 separated constituents in essential oil of Notoptergium incium , accounting for 92.13%, were identified and quantified by the developed method.

Key words: Gas chromatography mass spectrometry(GC MS), Evolving window orthogonal projection(EWOP), Orthogonal projection approach(OPA), Notoptergium incium (NI), Volatile oil

中图分类号:

O657

TrendMD:

郭方遒, 梁逸曾, 黄兰芳, 徐承建. GC-MS与正交投影法或渐进窗口正交投影法用于羌活挥发油成分的测定. 高等学校化学学报, 2004, 25(3): 430.

GUO Fang-Qiu, LIANG Yi-Zeng, HUANG Lan-Fang, XU Cheng-Jian . Determination of the Volatile Chemical Constituents of Notoptergium Incium by GC-MS and OPA or EWOP. Chem. J. Chinese Universities, 2004, 25(3): 430.

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