中红外光纤技术用于口腔肿瘤在体原位检测的研究

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (2): 348.

中红外光纤技术用于口腔肿瘤在体原位检测的研究

徐怡庄¹, 张元福¹, 杨丽敏¹, 彭歆², 孙开华², 李庆波¹, 周维金¹, 武惠中³, 俞光岩², 刘智¹, 翁诗甫¹, 王晶², 赵莹⁴, 徐端夫⁴, 吴瑾光¹

1. 北京大学化学与分子工程学院, 稀土材料化学及应用国家重点实验室, 北京 100871;
2. 北京大学口腔医学院, 北京 100081;
3. 北京第二光学仪器厂, 北京 100015;
4. 中国科学院化学研究所, 北京 100080

收稿日期:2003-08-28 出版日期:2004-02-24 发布日期:2004-02-24
通讯作者: 徐怡庄(1967年出生),男,博士,副教授,从事物理化学研究.E-mail;xyz@pku.edu.cn E-mail:xyz@pku.edu.cn
基金资助:
国家自然科学基金(批准号;39730160,50103001,20171004);国家重点基础研究发展规划项目(批准号;G1998061307,2002CCA01900)资助

In Vivo and Rapid Detection of the Malignancy Tumor of Oral Tissues Using Mid-FTIR Fiber Optics Spectroscopy

XU Yi-Zhuang¹, ZHANG Yuan-Fu¹, YANG Li-Min¹, PENG Xin², SUN Kai-Hua², LI Qing-Bo¹, ZHOU Wei-Jin¹, WU Hui-Zhong³, YU Guang-Yan², LIU Zhi¹, WENG Shi-Fu¹, WANG Jing², ZHAO Ying⁴, XU Duan-Fu⁴, WU Jin-Guang¹

1. State Key Laboratory of Rare Earth Materials and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
2. Medical School of Stomatology, Peking University, Beijing 100081, China;
3. Beijing Secondary Optical Instrument Factory, Beijing 100015, China;
4. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

Received:2003-08-28 Online:2004-02-24 Published:2004-02-24

摘要/Abstract

关键词: 中红外光纤, FTIR, 口腔肿瘤, 在体原位检测

Abstract: Our previous studies show that mid-FTIR spectroscopy can be used to distinguish malignant oral tissue from normal tissue under in vitro condition. Here, an in-situ FTIR spectroscopic measurement was performed to record FTIR spectra of normal and malignant oral tissues including salivary gland, tongue, parotid gland, submandibular gland etc. during clinical examination. The FTIR spectra of various oral tissues were acquired when an ATR probe linked to the FTIR spectrometer via mid-IR optical fibers was pressed on the tissues of the patients. For example, a patient(male, 76 years old) with tumor on the left parotid and the corresponding normal tissue on the right parotid were measured and obvious differences were observed. The spectral features of normal tissue and tumor are in good agreement with the criteria established in our previous work. (1) 1 389 cm^-1 band is quite strong in tumor, while the corresponding band in normal tissue is weaker than 1 452 cm^-1 band. (2) In normal tissue, 1 250 cm^-1 band is stronger, but the 1 250 cm^-1 band disappeared in the skin of malignant tissue. The above results demonstrate that in vivo FTIR spectra are in good agreement with our previous results obtained under in vitro condition. We believe that in vivo FTIR spectroscopy, providing the first-hand information concerning whether the suspected tissue is cancerous or not, is helpful for doctors in clinical activity.

Key words: Mid-IR fiber optics, FTIR, Malignancy tumor of oral tissue, In vivo and in situ detection

中图分类号:

O657.33

TrendMD:

徐怡庄, 张元福, 杨丽敏, 彭歆, 孙开华, 李庆波, 周维金, 武惠中, 俞光岩, 刘智, 翁诗甫, 王晶, 赵莹, 徐端夫, 吴瑾光. 中红外光纤技术用于口腔肿瘤在体原位检测的研究. 高等学校化学学报, 2004, 25(2): 348.

XU Yi-Zhuang, ZHANG Yuan-Fu, YANG Li-Min, PENG Xin, SUN Kai-Hua, LI Qing-Bo, ZHOU Wei-Jin, WU Hui-Zhong, YU Guang-Yan, LIU Zhi, WENG Shi-Fu, WANG Jing, ZHAO Ying, XU Duan-Fu, WU Jin-Guang . In Vivo and Rapid Detection of the Malignancy Tumor of Oral Tissues Using Mid-FTIR Fiber Optics Spectroscopy. Chem. J. Chinese Universities, 2004, 25(2): 348.

[1]	潘庆华, 徐怡庄, 来国桥, 刘少轩, 陈静, 杨丽敏, 张元福, 翁诗甫, 吴瑾光. 中红外光纤光谱法在化学反应过程中的应用[J]. 高等学校化学学报, 2012, 33(09): 1964.
[2]	赵瑾刘亚奇徐怡庄张元福吴瑾光侯宽永赵红梅雷玉涛周孝思徐智凌晓锋. 傅里叶变换红外光谱鉴别正常和良恶性乳腺组织[J]. 高等学校化学学报, 2011, 32(2): 246.
[3]	金兰英, 姜艳霞, 廖宏刚, 曾冬梅, 孙世刚. 红外光谱研究PEO基离子液体聚合物电解质[J]. 高等学校化学学报, 2009, 30(4): 767.
[4]	郑立仁,黄柏标,尉吉勇, . 碳辅助CVD制备氧化硅纳米线及其光学性能[J]. 高等学校化学学报, 2009, 30(2): 250.
[5]	潘庆华,王冰冰,来国桥,徐怡庄,张元福,吴奇光,孙开华,彭歆,俞光岩,刘玉峰,吴瑾光 . 傅里叶变换中红外光纤光谱法用于腮腺肿瘤的检测[J]. 高等学校化学学报, 2007, 28(5): 843.
[6]	徐怡庄,赵莹,凌晓锋,杨丽敏,徐智,周孝思,张元福,龚蓉晔,潘庆华,王冰冰,石景森,徐端夫,吴瑾光 . 胆囊疾病的临床快速诊断红外光谱新方法研究[J]. 高等学校化学学报, 2007, 28(4): 645.
[7]	陈青松, 李君涛, 周志有, 颜佳伟, 孙世刚 . 纳米结构钴薄膜电极的制备及其异常红外性能研究[J]. 高等学校化学学报, 2006, 27(8): 1500.
[8]	薛茹林种玉郑建红王琪颜长明陈翠雪叶青傅锦坤. Ag⁺生物吸附的谱学研究[J]. 高等学校化学学报, 2006, 27(3): 553.
[9]	曹希传,,张卓琦,Patel Ketan,Anderson W. Michael . 纯硅介孔分子筛SBA-15表面的手性移植 [J]. 高等学校化学学报, 2006, 27(11): 2013.
[10]	王琪,孙公权,闫世友,汪国雄,辛勤,陈青松,李君涛,姜艳霞,孙世刚 . PtRu/C电催化剂上甲醇吸附氧化过程的电化学原位红外光谱[J]. 高等学校化学学报, 2006, 27(11): 2123.
[11]	宋秀铎赵凤起刘子如潘清罗阳. 柠檬酸铋的热分解机理、非等温反应动力学及其对双基推进剂燃烧的催化作用[J]. 高等学校化学学报, 2006, 27(1): 125.
[12]	李君涛, 陈友江, 苏章菲, 周志有, 陈声培, 孙世刚. Pd微电极阵列表面纳米薄膜的方波电位制备及其特殊的红外性能[J]. 高等学校化学学报, 2005, 26(4): 710.
[13]	王立旭王德秋吴玉清. 溶液中聚(N-异丙基-2-甲基丙烯酰胺)热相变可逆性的杂合二维相关红外光谱研究[J]. 高等学校化学学报, 2005, 26(12): 2319.
[14]	许凯陈鸣才张秀菊刘红波. 原位FTIR光谱法研究联萘基环氧树脂体系的固化反应[J]. 高等学校化学学报, 2005, 26(12): 2377.
[15]	徐怡庄赵莹徐智杨丽敏刘玉峰凌晓锋张能维王立新王冰冰孟涛张元福翁诗甫周孝思徐端夫吴瑾光. 中红外光纤光谱法用于甲状腺肿物的体表、在体及离体检测研究[J]. 高等学校化学学报, 2005, 26(12): 2227.