高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (11): 2013-2015.

• 研究快报 • 上一篇    下一篇

产物可用电喷雾质谱检测的氨基酸/肽季铵化新方法

包慧敏1, 翟建军1,2, 张国安1, 杨芃原1, 陈先1   

  1. 1. 复旦大学化学系, 上海200433;
    2. 兰州大学化学系, 兰州730000
  • 收稿日期:2004-05-17 出版日期:2004-11-24 发布日期:2004-11-24
  • 基金资助:

    国家重点基础研究发展计划项目(批准号:001CB510202);国家高技术研究发展计划项目(批准号:2001AA233031);国家自然科学基金(批准号:20299030)资助

A Novel Method for Quaternization of Amino Acid/Peptide and Its Product Detection Using Electrospray Ionization Mass Spectrometry

BAO Hui-Min1, ZHAI Jian-Jun1,2, ZHANG Guo-An1, YANG Peng-Yuan1, CHEN Xian1   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433, China;
    2. Department of Chemistry, Lanzhou University, Lanzhou 730000, China
  • Received:2004-05-17 Online:2004-11-24 Published:2004-11-24

关键词: 电喷雾离子化质谱, 氨基酸/肽, 季铵化

Abstract: A novel detection method for quaternization of amino acid and peptide was reported. In this method Ag2CO3 was employed instead of KHCO3 to catalyze the occurrence of quaternization reaction. The absence of metal ions in the reaction system enabled us to in situ detect the quaternizing products by using electrospray ionization mass spectrometry(ESI-MS). We analyzed quaternizing products of six amino acids and two peptides in situ, and obtained the ratio of major product to by-product, which suggests the new method possesses prominent advantages over the old one. This method could offer a potential chance to combine quaternization with mass spectrometry which is helpful for the future use of quaternization reaction in quantitative proteomics.

Key words: Electrospray ionization mass spectrometry, Amino acid/peptide, Quaternization

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