含对溴苯基的新型DNA切割剂的合成及活性研究

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (8): 1430.

含对溴苯基的新型DNA切割剂的合成及活性研究

李丽华, 刘丹, 汤菲力, 袁谷

北京大学化学与分子工程学院化学生物学系, 生物有机与分子工程教育部重点实验室, 北京 100871

收稿日期:2002-08-29 出版日期:2003-08-24 发布日期:2003-08-24
通讯作者: 袁谷(1953年出生),男,博士,教授,博士生导师,主要从事生物有机化学研究.E-mail:guyuan@pku.edu.cn E-mail:guyuan@pku.edu.cn
基金资助:
国家自然科学基金(批准号:39970169,29872001)资助

Synthesis and Cleaving Activity of DNA Photocleaver Containing Bromoacetophenone

LI Li-Hua, LIU Dan, TANG Fei-Li, YUAN Gu

Key Laboratory of Bioorganic and Molecular Engineering of Education Ministry, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

Received:2002-08-29 Online:2003-08-24 Published:2003-08-24

摘要/Abstract

关键词: 寡聚酰胺, DNA识别分子, DNA切割剂

Abstract: Anovel DNA photocleaver containing bromoacetophenone-polyamide was synthesized by DCC coupling reaction in the presence of DMAP, and its DNA cleaving activity was investigated by using pBluescript SK DNA by method of gelelectrophoresis.The result shows that the photocleaver containing bromoacetophenone-polyamide has a remarkable cleaving activity for pBluescript SK DNA.

Key words: Polyamide, DNA recognition molecules, DNA-photocleavage agents

中图分类号:

O629.7

TrendMD:

李丽华, 刘丹, 汤菲力, 袁谷. 含对溴苯基的新型DNA切割剂的合成及活性研究. 高等学校化学学报, 2003, 24(8): 1430.

LI Li-Hua, LIU Dan, TANG Fei-Li, YUAN Gu . Synthesis and Cleaving Activity of DNA Photocleaver Containing Bromoacetophenone. Chem. J. Chinese Universities, 2003, 24(8): 1430.