高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (7): 1198.

• 研究快报 • 上一篇    下一篇

以pH敏感相分离高分子为载体的酶联荧光免疫分析法测定人IgG

林鹏1,2, 郑洪1, 杨黄浩1, 杨薇1, 张长弓3, 许金钩1   

  1. 1. 厦门大学化学系, 分析科学教育部重点实验室, 厦门 361005;
    2. 集美大学水产学院, 厦门 361021;
    3. 厦门大学抗癌研究中心, 厦门 361005
  • 收稿日期:2003-04-01 出版日期:2003-07-24 发布日期:2003-07-24
  • 通讯作者: 许金钩(1937年出生),男,教授,博士生导师,从事分子发光分析研究.E-mail:jgxu@xmu.edu.cn E-mail:jgxu@xmu.edu.cn
  • 基金资助:

    教育部博士学科点基金(批准号:2000038404);福建省自然科学基金(批准号:D0110002)资助

Enzyme-linked Fluorescence Immunoassay for Human IgG by Using a pH-Sensitive Phase Separating Polymer as Carrier

LIN Peng1,2, ZHENG Hong1, YANG Huang-Hao1, YANG Wei1, ZHANG Chang-Gong3, XU Jin-Gou1   

  1. 1. Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, China;
    2. Aquaculture College, Jimei University, Xiamen 361021, China;
    3. Cancer Research Center, Xiamen University, Xiamen 361005, China
  • Received:2003-04-01 Online:2003-07-24 Published:2003-07-24

PDF (PC)

被引次数

18

关键词: pH敏感相分离高分子, 酶联荧光免疫分析, 人IgG

Abstract: Poly(N-isopropylacrylamide-N,N-dimethyl aminopropyl methacrylamide) [P(NIP-DMAPM)], a novel linear water-soluble pH-sensitive polymer whose phase transition pH is about 7.4 at 37 ℃, was synthesized. P(NIP-DMAPM) polymer precipitated out of water above a critical pH 7.4 at 37 ℃ and redissolved at pH below 7.4. P(NIP-DMAPM) was used as a novel separation carrier for the reactants in immunoassay and a enzyme-linked fluorescence immunoassay for human IgG was built. The linear range of determination was 0-1700 ng/mL and the limit of detection was 2.2 ng/mL. The sensitivity of this method was close to that of traditional ELISA by using same reactants. However, the assay was much faster. This method has been applied to the determination of the human IgG levels in human serum with satisfactory results.

Key words: pH-Sensitive phase separating polymer, Enzyme-linked fluorescence immunoassay, Human IgG

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