高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (5): 840.

• 研究简报 • 上一篇    下一篇

在对映体水平上测定土壤中烯效唑的方法

李朝阳, 张智超, 刘怡姗, 周其林, 高如瑜, 王琴孙   

  1. 南开大学元素有机化学研究所元素有机化学国家重点实验室, 天津 300071
  • 收稿日期:2002-02-26 出版日期:2003-05-24 发布日期:2003-05-24
  • 通讯作者: 张智超(1969年出生),男,博士.副教授,从事有机分析化学和有机污染化学研究.E-mail:zczhang@nankai.edu.cn E-mail:zczhang@nankai.edu.cn
  • 基金资助:

    国家自然科学青年基金(批准号:29907002);教育部高等学校骨干教师资助计划资助

Determination of Uniconazole in Soil with an Enantioselective Way

LI Zhao-Yang, ZHANG Zhi-Chao, LIU Yi-Shan, ZHOU Qi-Lin, GAO Ru-Yu, WANG Qin-Sun   

  1. State Key Laboratory of Elemento-Organic Chemistry, Institute of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China
  • Received:2002-02-26 Online:2003-05-24 Published:2003-05-24

PDF (PC)

被引次数

14

摘要： 目前使用的农药中约25％是手性化合物.手性农药对映体的生物活性往往有很大差异,例如常用的三唑类植物生长调节剂烯效唑(Uniconazole),其S体的活性是R体的7倍.但目前绝大多数手性农药是以消旋体形式使用的.对于手性农药,目前的研究工作主要集中在其作为农药的生物活性的对映体选择性上,而对其环境行为的对映体选择性研究则很少,缺乏在对映体水平上测定环境介质.

关键词: 手性农药, 烯效唑, 对映体比值, 底物固相分散, 手性高效液相色谱

Abstract: Afast and precise method was developed for the determination of the enantiomers of chiral pes-ticidc-uniconazole in soil. Matrix solid-phase dispersion (MSPD) technology was used to extract uniconazole from soil. MSPDconditions were optimized stepwise. The MSPD extract was directly used for quantitative determination of uniconazole by silica-based high performance liquid chromatography(HPLC) with UVdetection. The recoveries of uniconazole from three types of soils fortified at three levels of 0. 1, 1, 10 μg/g ranged from 75%-97% with RSDs<7%. On this basis, uniconazole was further isolated from the remainder of MSPD extract by silica-based HPLC and then subjected to chiral HPLCto determine its enan-tiomcric ratio. The determined ERs in the soils spiked with racemic uniconazole agreed sufficiently well with those in the standard solutions within the error limit of the method.

Key words: Chiral pesticide, Uniconazole, Enantiomeric ratio, Matrix solid-phase dispersion, Chiral HPLC

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