电化学诱导咪唑铜配合物断裂DNA的研究

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (3): 414.

电化学诱导咪唑铜配合物断裂DNA的研究

赵广超, 朱俊杰, 陈洪渊

南京大学化学系, 分析科学研究所, 介观材料实验室, 南京 210093

收稿日期:2002-01-10 出版日期:2003-03-24 发布日期:2003-03-24
通讯作者: 陈洪渊(1937年出生),男,教授,博士生导师,中国科学院院士,主要从事电分析化学研究.
基金资助:
教育部博士学科点基金(批准号:29835110,90206037)资助

Studies on Electrochemically Induced DNA Cleavage by Imidazole Copper Complex

ZHAO Guang-Chao, ZHU Jun-Jie, CHEN Hong-Yuan

Department of Chemistry, Institute of Analysis Science, Lab. of Mesoscopic Materials Science, Nanjing University, Nanjing 210093, China

Received:2002-01-10 Online:2003-03-24 Published:2003-03-24

摘要/Abstract

摘要： 二价咪唑铜配合物可以在电极表面发生氧化还原反应,该过程对应于单电子准可逆过程.荧光光谱电化学结果表明,还原产物一价咪唑铜配合物可以被空气中的O₂氧化为二价.在中性的Tris-HCl缓冲溶液中,配合物与DNA之间存在弱相互作用,其键合常数仅为2.7×10²L/mol.荧光光谱及电化学结果表明为嵌入式作用.据此建立了一种电化学诱导咪唑铜配合物对DNA断裂的新方法,并采用差示扫描量热(DSC)法检验了断裂结果.

关键词: 电化学诱导, 咪唑铜配合物, DNA断裂

Abstract: There are a couple of quasi-reversible redox peaks of Cu(Ⅱ)-imidazole[Cu(Ⅱ)Im₄] with one electron process on Pt electrode. Fluorescence spectroelectrochemical experiments reveal that the Cu(Ⅰ) complex reduced by electrochemistry can be reoxidized by O₂ in air. In neutral Tris-HCl buffer, {Cu(Ⅱ)Im₄} can bind to DNA with the binding constant of 2.7×10 2 L/mol. Obviously, this is a weak interaction. However, it is an intercalative model which has been identified by the experiments of fluorescence and electrochemistry. A novel method for the cleavage of DNA by the electrochemically-induced process of the reduction of Cu(Ⅱ)Im₄ is established and differential scanning calorimetries(DSC) are used to detect the results of DNA cleavage.

Key words: Electrochemical induction, Cu(Ⅱ)-imidazole complex, DNA cleavage

中图分类号:

O657

TrendMD:

赵广超, 朱俊杰, 陈洪渊. 电化学诱导咪唑铜配合物断裂DNA的研究. 高等学校化学学报, 2003, 24(3): 414.

ZHAO Guang-Chao, ZHU Jun-Jie, CHEN Hong-Yuan . Studies on Electrochemically Induced DNA Cleavage by Imidazole Copper Complex. Chem. J. Chinese Universities, 2003, 24(3): 414.