高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (2): 255.

• 论文 • 上一篇    下一篇

新型有机荧光染料嵌合的核壳荧光纳米材料的研制

段菁华, 王柯敏, 谭蔚泓, 何晓晓, 何春梅, 刘斌, 李杜, 黄杉生, 羊小海, 莫远尧   

  1. 湖南大学化学化工学院, 生物技术研究院, 化学生物传感与计量学国家重点实验室, 长沙 410082
  • 收稿日期:2002-07-22 出版日期:2003-02-24 发布日期:2003-02-24
  • 通讯作者: 王柯敏(1957年出生),男,博士,教授,博士生导师,从事纳米和单分子水平上的生化分析研究.E-mail:kmwang@hnu.net.cn E-mail:kmwang@hnu.net.cn
  • 基金资助:

    国家自然科学基金重点项目(批准号:20135010);国家杰出青年基金(批准号:29825110);教育部重大项目基金(批准号:2000-156);海外青年学者合作研究基金(批准号:20028506);湖南省自然科学基金(批准号:00GKY1011);湖南省基金(

A Study of a Novel Organic Fluorescent Core-shell Nanoparticle

DUAN Jing-Hua, WANG Ke-Min, TAN Wei-Hong, HE Xiao-Xiao, HE Chun-Mei, LIU Bin, LI Du, HUANG Shan-Sheng, YANG Xiao-Hai, MO Yuan-Yao   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering, Institute of Biological Technology, Hunan University, Changsha 410082, China
  • Received:2002-07-22 Online:2003-02-24 Published:2003-02-24

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被引次数

86

摘要： 采用油包水的反相微乳液方法,首次以羊抗人免疫球蛋白(IgG)标记的异硫氰酸荧光素(FITC)为核材料,成功地制备了FITC的核壳荧光纳米颗粒,克服了采用传统方法制备核壳荧光纳米颗粒中存在的荧光染料泄露的问题.制备的这种核壳荧光纳米颗粒比细胞小很多,且具有生物亲和性,可为纳米生物传感器件提供新型材料.基于该核壳荧光纳米颗粒的标记方法也为生物医学提供了一种新型的非同位素分析方法.

关键词: 油包水微乳液, 异硫氰酸荧光素(FITC), 羊抗人免疫球蛋白(IgG), 核壳荧光纳米颗粒

Abstract: A novel fluorescent core-shell nanoparticle has been developed by encapsulating the fluorophore into silica shell with a reverse microemulsion technique. The FITC encapsulation is greatly enhanced by conjucting a water-soluble protein IgG which significantly reduces FITC leaching from the nanoparticles when immersed inside water. The FITC leaching problem of the nanoparticles prepared with IgG has been much less severe than that with the FITC nanoparticles prepared with unmodified dye or dye modified with dextrans of higher molecular weight. The fluorescent core-shell nanoparticles are also pH sensible. They can be used as pH optical sensors inside cell phagocytosed by murine macrophages.

Key words: Water-in-oil microemulsion, Fluorescein isothiocyante(FITC), Immunoglobal(IgG), Fluorescent core-shell nanoparticles

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