高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (10): 1733.

• 论文 •    下一篇

细胞色素c氧化酶可溶性结构域中Val162突变对蛋白结构和功能的影响

宋爱新, 李连之, 黄仲贤   

  1. 复旦大学化学系, 化学生物学实验室, 上海 200433
  • 收稿日期:2002-12-18 出版日期:2003-10-24 发布日期:2003-10-24
  • 通讯作者: 黄仲贤(1940年出生),男,教授,博士生导师,从事生物无机化学和蛋白质化学研究.E-mail:zxhuang@fudan.edu.cn E-mail:zxhuang@fudan.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:39990600)资助

Effect of Val162 Mutation on Structure and Function of Cytochrome c Oxidase Soluble Domain

SONG Ai-Xin, LI Lian-Zhi, HUANG Zhong-Xian   

  1. Chemistry and Biology Lab, Department of Chemistry, Fudan University, Shanghai 200433, China
  • Received:2002-12-18 Online:2003-10-24 Published:2003-10-24

PDF (PC)

被引次数

5

摘要： 采用蛋白质工程的定点突变技术和UV-Vis,CD,荧光,EPR及快速动力学技术考察了与癌症相关的Val162的突变对细胞色素c氧化酶可溶性结构域的影响.研究发现,该突变导致了蛋白二级结构的转化和电子传递活性的降低,这为理解其致病机制提供了化学基础信息.

关键词: 细胞色素c氧化酶, 点突变与疾病, 结构转化, 电子传递

Abstract: The effect of Val162 mutation(potentially relative to cancer) on cytochrome coxidase soluble Cu Adomain was studied by site-directed mutagenesis technique combined with UV-Vis, CD, fluorescence spectra, EPR and rapid kinetic methods.It is found that the mutation at the Val162site leads to a structural transformation and decreasing electron transfer activity.It provides important information for understanding the pathological mechanism.

Key words: Cytochrome coxidase, Site-directed mutation and diseases, Structure transformation, Electron transfer

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