高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (9): 1692.

• 研究简报 • 上一篇    下一篇

环境样品中生物可利用态镉的反相离子对-HPLC-荧光检测

李彬, 王秋泉, 严华, 杨利民, 黄本立   

  1. 厦门大学化学系教育部现代分析科学重点实验室, 厦门 361005
  • 收稿日期:2001-09-05 出版日期:2002-09-24 发布日期:2002-09-24
  • 通讯作者: 黄本立(1925年出生),男,教授,博士生导师,从事原子光谱/质谱分析研究.E-mail:BLH@xmu.edu.cn E-mail:BLH@xmu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20175019);福建省自然科学基金(批准号:F00011)资助

Determination of Bioavailable Cadmium in Environmental Samples by IP-RP-HPLC Coupled with On-line Fluorescence Assay

LI Bin, WANG Qiu-Quan, YAN Hua, YANG Li-Min, HUANG Ben-Li   

  1. Department of Chemistry & the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China
  • Received:2001-09-05 Online:2002-09-24 Published:2002-09-24

PDF (PC)

被引次数

5

关键词: 反相离子对色谱, 生物可利用态镉, 十六烷基三甲基溴化铵, 8-羟基喹啉-5-磺酸

Abstract: Anovel reserved-phase ion-pair liquid chromatographic method using hexadecyltrimethylammonium bromide(HTMAB) as an ion-pair reagent for separating the complexes of metal and 8-hydroxyquinoline-5-sulphonic acid(HQS) coupled with on-line fluorescence detection has been developed. The chromatographic behavior of metal-HQScomplexes in the proposed system was systematically investigated.When the C18 column modified by HTMABand the suitable mobile phase coupled with fluorescence detection(λex=388 nm; λem=518 nm) were employed, the detection limit of cadmium is 7.72 ng/mLwith an injection volume of 20 μL, and the linear range of calibration curve for cadmium is 50—800 ng/mL. It was successfully applied to determination of bio-available cadmium in polluted soils of China. Moreover, the possible species of Cd-HQSon the column has been investigated by log-log plot analysis.

Key words: Reversed-phase ion-pair HPLC, Bio-available cadmium, Hexadecyl trimethyl ammonium bromide, 8-Hydroxyquinoline-5-sulphonic acid

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