高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (6): 1073.

• 研究论文 • 上一篇    下一篇

稻秆木素侧链13C同位素示踪及固体13CNMR分析

顾瑞军1, 谢益民1, 曾绍琼1, 伍红1, 安田征市2   

  1. 1. 华南理工大学制浆造纸工程国家重点实验室, 广州 510640;
    2. 日本名古屋大学农学部, 名古屋464-8601
  • 收稿日期:2001-03-09 出版日期:2002-06-24 发布日期:2002-06-24
  • 通讯作者: 谢益民(1965年出生),男,博士,教授,主要从事植物资源化学研究.E-mail:ppymxie@scut.edu.cn E-mail:ppymxie@scut.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29974011);霍英东教育基金(批准号:71066);广东省千百十工程优秀人才基金资助

Carbon-13 Enrichment of Rice Stalk Lignin Traced by Solid State 13C NMR Spectroscopy

GU Rui-Jun1, XIE Yi-Min1, ZENG Shao-Qiong1, WU Hong1, Seiichi-Yasuda2   

  1. 1. State Key Lab. of Pulp & Paper Engineering, South China University of Technology, Guangzhou 510640,China;
    2. School of Agricultural Science, Nagoya University, Nagoya University, Nagoya 464-8601, Japan
  • Received:2001-03-09 Online:2002-06-24 Published:2002-06-24

摘要: 在水稻(Oryza sativa L.)生长过程中,向其茎秆部节间的空腔分别注入在侧链α,β,γ位带有13C标记的松柏醇葡萄糖甙,得到13C标记的稻秆木素,用高分辨率固体核磁13CNMR对其组织进行分析,发现外源性的松柏醇葡萄糖甙并不影响水稻中木素的正常合成.证明了β-O-4,β-β,β-5和β-1结构是稻秆原本木素中的主要结构,另外还有少量的松柏醇和阿魏酸类结构,并证实木素在α位与糖类等组分有共价键形成.

关键词: 稻秆, 木素, 13C同位素示踪, 固体13CNMR

Abstract: Carbon-13 enrichment of specific carbon of rice stalk lignin was achieved by feeding coniferin-[ α-13C], coniferin-[ β-13C] and coniferin-[ γ-13C] into an internode cavity of the stalk of a growing rice plant(Oryza sativa L.). The resulting rice stalk tissues were analyzed by high-resolution solid state carbon-13 nuclear magnetic resonance (13C NMR) spectroscopy. It was found that the administration did not affect the normal ligninfication of rice plant. It was also proved that β-O-4, β-β, β-5 and β-1 were mainly a substructure in protolignin of rice stalk although there were some minor substructures as coniferyl alcohol and ferulic acid unit. Importantly, the result indicated that significant bonding occurred at α-position to other constituents, presumed to be mainly of carbohydrates.

Key words: Rice stalk, Lignin, Carbon-13 isotopic tracer, Solid state 13 CNMR spectroscopy

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