高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (5): 809.

• 研究论文 • 上一篇    下一篇

加速器质谱法研究尼古丁与小鼠血液蛋白的加合作用

王艳1, 王海芳1, 孙红芳1, 王祥云1, 刘元方1, 李坤2, 刘克新2, 鲁向阳2, 马宏骥2   

  1. 1. 北京大学化学与分子工程学院, 北京 100871;
    2. 北京大学物理学院重离子物理研究所, 北京 100871
  • 收稿日期:2001-03-06 出版日期:2002-05-24 发布日期:2002-05-24
  • 通讯作者: 王海芳(1969年出生),女,硕士,副教授,从事生物加速器质谱应用研究.
  • 基金资助:

    国家自然科学基金(批准号:199350220);北京大学重离子物理教育部重点实验室资助.

Accelerator Mass Spectrometry Studies on Adduction of Nicotine with Hemoglobin and Serum Albumin in Mouse

WANG Yan1, WANG Hai-Fang1, SUN Hong-Fang1, WANG Xiang-Yun1, LIU Yuan-Fang1, LIU Ke-Xin2, LU Xiang-Yang2, MA Hong-Ji2, LI Kun2   

  1. 1. College of Chemistry and Molecular Engineering;
    2. Institute of Heavy Ion Physics, School of Physics, Peking University, Beijing 100871, China
  • Received:2001-03-06 Online:2002-05-24 Published:2002-05-24

PDF (PC)

被引次数

6

摘要： 采用加速器质谱法(AMS)研究了小鼠血液中的血红蛋白(Hb)、白蛋白(SA)和尼古丁的加合作用.在尼古丁剂量从相应于人吸烟的环境剂量水平0.1μg/kg体重到100μg/kg体重范围内,测量了尼古丁－血红蛋白加合物和尼古丁－白蛋白加合物的剂量效应曲线.两种蛋白的尼古丁加合物水平都随着尼古丁剂量的升高而线性增加.在实验剂量范围内,两种蛋白质加合物的水平与小鼠肝DNA加合物水平也呈现良好的线性关系.这说明两种蛋白质加合物均可以替代DNA加合物作为尼古丁的生物标记物.在本工作中,蛋白质加合物的测量灵敏度为0.03pmol/g蛋白(即3.2个加合物/1012氨基酸残基),达到了文献报道的测量蛋白质生物标记物最高的灵敏度.

关键词: 加速器质谱法(AMS), 尼古丁, 血红蛋白加合物, 白蛋白加合物

Abstract: Adducts of hemoglobin(Hb) and serum albumin(SA) with nicotine in mouse blood were mea-sured respectively by using accelerator mass spectrometry (AMS).The dose response of adduct levels ofHb/SAand the correlative relationship between the two blood protein adducts were investigated over arange of doses(0.1-100.0 μg/kg body weight), which were equivalent to a low-level dose of environ-mental exposure of cigarette smoking.Both the levels of Hb and SAadducts increased with increasing doseand showed a linear relationship.By plotting Hb/SAadducts versus DNAadducts, a linear relationship fitsthe experimental observations best, which means that Hb/SAadduct could substitute for DNAadduct as abiomarker for nicotine.The detection limit of protein adduct in this work is 0.03 pmol/g protein(namely3.2 adducts/1012 amino acid residues), the excellent sensitivity in protein biomarker measurements com-pared to the data reported previously.

Key words: Accelerator mass spectrometry(AMS), Nicotine, Hemoglobin (Hb) adduct, Serum albumin (SA) adduct

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