高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (4): 552.

• 研究论文 • 上一篇    下一篇

鼠脑尾核和海马区微透析液中儿茶酚胺类神经递质的高效液相色谱分析

常理文1, 徐桂芸1, 韩慧婉1, 常溪燕2, 赵卓1   

  1. 1. 中国科学院化学研究所分子科学中心, 北京 100080;
    2. 北京石油化工科学研究院, 北京 100083
  • 收稿日期:2001-03-21 出版日期:2002-04-24 发布日期:2002-04-24
  • 通讯作者: 常理文(1938年出生),男,研究员,从事生化物质的色谱和毛细管电泳研究.E-mail:Chang-Liwen@263.net E-mail:Chang-Liwen@263.net
  • 基金资助:

    国家自然科学基金(批准号:29775027);中国科学院基础性研究重大项目(批准号:KJ－951－A1－507－03)资助.

High Performance Liquid Chromatographic Analysis of Catecholaminein the Microdialysate from Rat Brain

CHANG Li-Wen1, XU Gui-Yun1, HAN Hui-Wan1, CHANG Xi-Yan2, ZHAO Zhuo1   

  1. 1. Institute of Chemistry, Center for Molecular Science, Chinese Academy of Sciences, Beijing 100080, China;
    2. Beijing Research Institute of Petroleum Processing, Beijing 100083, China
  • Received:2001-03-21 Online:2002-04-24 Published:2002-04-24

PDF (PC)

被引次数

16

摘要： 采用反相液相色谱分离-脉冲安培检测和微透析取样技术,测定了取自鼠脑尾核和海马区微透析液中儿茶酚胺类神经递质的含量。对不同神经活动状态下取得微透析液中肾上腺素含量作了比较,将该法用于测定肾上腺素,灵敏度为25μg/L.

关键词: 神经递质, 微透析取样, 脉冲安培检测

Abstract: A method for determination of catecholamine and related metabolite in the microdialysate fromrat brain by HPLC With pulsed amperometric detection was described.The optimum condition of chro-matographic separation and pulsed amperometri detection were studied.Sampling was carried out by mi-crodialysis technique from rat brain.Acomparison of epinephrine(E) content in microdialysates from ratbrain at various event was made.The detection limit of the method is 25 μg/L for epinephrine.

Key words: Catecholamine, Microdialysis, Pulsed amperometric detection

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