高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (2): 199.

• 研究论文 • 上一篇    下一篇

高效毛细管电泳分离/电导检测麻黄碱和伪麻黄碱

郑一宁1, 谢天尧1, 莫金垣1, 韦寿莲2   

  1. 1. 中山大学化学系, 广州 510275;
    2. 广西民族学院化学化工系, 南宁 530006
  • 收稿日期:2001-01-08 出版日期:2002-02-24 发布日期:2002-02-24
  • 通讯作者: 莫金垣(1934年生),男,教授,博士生导师,主要从事电分析化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29675033);广东省自然科学基金(批准号:001237)资助

The Ephedrine and Pseudoephedrine Separation with the Capillary Electrophoresis and Determination by Conductivity Detector

ZHENG Yi-Ning1, XIE Tian-Yao1, MO Jin-Yuan1, WEI Shou-Lian2   

  1. 1. Department of Chemistry, Zhongshan University, Guangzhou 510275, China;
    2. Department of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China
  • Received:2001-01-08 Online:2002-02-24 Published:2002-02-24

PDF (PC)

被引次数

77

摘要： 采用高效毛细管电泳电导检测法分离麻黄碱和伪麻黄碱,初步探讨了分离机理,建立了检测方法.以柠檬酸-柠檬酸钠为缓冲体系,铜盐为络合剂,在pH值为4.5、电压13.5kV的条件下,盐酸麻黄碱和伪麻黄碱得到了较好的分离,加入适量乙醇可改善峰形和分离效果.用该法以水杨酰胺为内标,对含盐酸麻黄碱和伪麻黄碱的实际样品进行检测,回收率为97.3%～101.1%,结果令人满意.

关键词: 高效毛细管电泳, 电导法, 盐酸麻黄碱, 伪麻黄碱

Abstract: The separation between ephedrine and pseudoephedrine with capillary electrophoresis and determination by the conductivity detector is reported. The separation mechanism is studied and the determination method is found. The effects of complex reagent concentration, concentration and pHof the running buffer, separation voltage and the organic solvent concentration are investigated and discussed. At 13.5 kVhigh voltage the ephedrine and pseudoephedrine are separated successfully in a citric acid sodium citrate buffer(pH=4.5) with the copper salt as the complex reagent. Ethanol is used to improve the peak shapes and separation effects. There are an excellent linearity between peak area and concentration of analytes in the concentration range of 1.4-165 mg/Lfor ephedrine and 0.825-165 mg/Lfor pseudophedrine with the detection limits(S/N =3) of 0825 and 0198 for ephedrine and pseudophedrine respectively. Relative standard deviations(RSD) of the ephedrine and psudoephedrine are 32%(ephedrine) and 28%(psudoephedrine) based on the determination of seven times. The ephedrine and pseudoephedrine in collunarium are detected and the satisfaction results are obtained.

Key words: Capillary electrophoresis, Conductivity detector, Ephedrine, Pseudoephedrine

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