高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (12): 2247.

• 研究简报 • 上一篇    下一篇

提高MALDI-MS测量溶菌酶分子量灵敏度的研究

石磊1,2, 王勇1, 刘志强1, 刘淑莹1   

  1. 1. 中国科学院长春应用化学研究所, 长春130022;
    2. 吉林大学公共卫生学院, 长春130021
  • 收稿日期:2001-09-07 出版日期:2002-12-24 发布日期:2002-12-24
  • 通讯作者: 刘淑莹(1943年出生),女,博士,研究员,博士生导师,从事质谱研究.

High-sensitivity Molecular Mass Determination of Lysozyme Prior to MALDI-MS

SHI Lei1,2, WANG Yong1, LIU Zhi-Qiang1, LIU Shu-Ying1   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. Public Health College, Jilin University, Changchun 130021, China
  • Received:2001-09-07 Online:2002-12-24 Published:2002-12-24

关键词: 基质辅助激光解吸电离质谱, 溶菌酶, 硝酸纤维素膜

Abstract: Sodium dodecyl sulfate(SDS) is a powerful solubilizing detergent which is often used during the separation of highly complex protein mixtures by one- or two-dimensional(2D) gel electrophoresis. Matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS) is a widely used technique for mass spectrometric analysis of some protein molecules compared to other techniques. But the presence of SDS or some salts usually leads to signal deterioration when using MALDI-MS. A method for using nitrocellulose membrane as the solid-phase carrier combined with n-octyl-β-D-glucopyranoside in the matrix highly enhances the sensitivity of the molecular mass determination of lysozyme. This technique has the advantage that the signal-to-noise of the molecular weight profile is improved compared with the mass spectrum and the profile is relatively easy to interpret.

Key words: MALDI-MS, Lysozyme, Nitrocellulose membrane

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