高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (11): 2070.

• 研究简报 • 上一篇    下一篇

蛋白A连续床亲和毛细管色谱柱的制备及性能考察

潘再法1, 莫卫民1, 吴仁安2, 罗权舟2, 叶明亮2, 邹汉法2   

  1. 1. 浙江工业大学化工学院, 杭州310014;
    2. 中国科学院大连化学物理研究所, 国家色谱研究分析中心, 大连116011
  • 收稿日期:2001-07-02 出版日期:2002-11-24 发布日期:2002-11-24
  • 通讯作者: 邹汉法(1961年出生),男,博士,研究员,博士生导师,主要从事生物液相色谱、毛细管电色谱的研究.
  • 基金资助:

    国家杰出青年基金(批准号:29725512)资助

Preparation and Characterization of Protein A Immobilized on Monolithic Capillary Column for Affinity Chromatography

PAN Zai-Fa1, MO Wei-Min1, WU Ren-An2, LUO Quan-Zhou2, YE Ming-Liang2, ZOU Han-Fa2   

  1. 1. College of Chemical Engineering, Zhejiang Uniersity of Technology, Hangzhou 310014, China;
    2. National Chromatographic R. & A. Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China
  • Received:2001-07-02 Online:2002-11-24 Published:2002-11-24

PDF (PC)

被引次数

17

关键词: 亲和色谱法, 连续床毛细管色谱柱, 蛋白A, 人免疫球蛋白G

Abstract: An assay technique for the determination of the human IgG in human plasma has been developed by utilizing protein A immobilized monolithic capillary column. The affinity chromatography experiment was performed on a capillary electrophoresis instrument by using its pressure system as the driving force. Reactive monolithic capillary columns have been prepared by in-situ copolymerization of the monomers in the presence of porogenic dilute, and protein A was immobilized on the monolithic rods directly or through a 11 carbon atom space arm with molded synthetic methods. The non-specific adsorption of two kinds of media has been studied and the results showed that the medium without space arm gives very low non-specific adsorption of BSA. The affinity column without space arm was used to determine the human immunoglobulin G(HIgG) in human serum. The correlative coefficient of the calibration curve reaches 0.998 7. The total time of rapid analysis was less than 0.7 min. The consumption of eluent buffer and sample is much low than by conventional HPLC.

Key words: Affinity chromatography, Monolithic capillary column, Protein A, Human immunoglobulin G

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