高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (1): 71.

• 研究论文 • 上一篇    下一篇

Pseudomonas stutzeri 1317合成中长链聚羟基脂肪酸酯的组成分析模型

徐军1, 贺文楠1, 郭宝华1, 胡平1, 张增民1, 张广2, 林会兰2, 陈金春2, 陈国强2   

  1. 1. 清华大学材料科学与工程研究院, 化工系高分子研究所, 北京 100084;
    2. 清华大学生物科学与技术系, 北京 100084
  • 收稿日期:2000-06-27 出版日期:2002-01-24 发布日期:2002-01-24
  • 通讯作者: 张增民(1936年出生),男,教授,博士生导师,从事高分子材料的共混改性和新型生物高分子材料合成和改性研究.
  • 基金资助:

    国家“九五”攻关基金(批准号:96-C03-03-02);清华大学基金资助

A Mathematical Model for Biosynthesis of Medium-chain-length Polyhydroxyalkanoates by Pseudomonas stutzeri1317

XU Jun1, HE Wen-Nan1, GUO Bao-Hua1, HU Ping1, ZHANG Zeng-Min1, ZHANG Guang2, LIN Hui-Lan2, CHEN Jin-Chun2, CHEN Guo-Qiang2   

  1. 1. Institute of Polymer Science & Engineering, Department of Chemical Engineering, School of Materials Science and Engineering, Tsinghua Unviversity, Beijing 100084, China;
    2. Department of Biological Science & Technology, Tsinghua University, Beijing 100084, China
  • Received:2000-06-27 Online:2002-01-24 Published:2002-01-24

摘要: Pseudomonasstutzeri 1317可以在不同碳源上生长,合成不同共聚单体单元组成的中长链聚羟基脂肪酸酯(mclPHAs).本文在已知的代谢途径的基础上,提出了关于mclPHAs组成的数学模型,结果表明,mclPHAs的共聚单体单元组成主要取决于前体生成途径,以及该途径和聚合途径酶对不同碳原子数前体的选择性.实验结果与文献报道结果均验证了该模型.代谢控制分析的结果表明,碳数为8~10的前体的聚合效率最高.通过调制前体生成途径,可以有效调节PHA的单体单元组成.

关键词: 中长链聚羟基脂肪酸酯(mclPHAs), Pseudom onas stutzeri, 数学模型, 单体单元组成, 代谢控制分析

Abstract: When grown on various carbon sources, P. stutzeri 1317 strain accumulates medium-chain-length polyhydroxyalkanoates(mcl PHAs) of various monomer composition. Amathematical model was developed to investigate what affects mcl PHAcomposition and how to tailor design the copolymer, and some interesting conclusions were deduced: the monomer composition was independent of cultivation time and the enzyme level; the content variance of different monomers resulted only from the selectivity ratio of the polymerase to the enzymes involved in theprecursor formation pathway; modulation of precursor formation pathway led to convenient manipulation of PHA monomer composition. The experimental results from several Pseudomonas strains proved the model an effective tool for tailor-design of mcl PHA copolymers.

Key words: Medium-chain-length polyhydroxyalkanoates(mcl PHAs), Pseudomonas stutzeri, Mathematical model, Monomer composition, Metabolic control analysis

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