基于Fmoc策略的O-磷酸化多肽化学合成研究

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (S1): 106.

基于Fmoc策略的O-磷酸化多肽化学合成研究

陈水冰, 李艳梅, 赵刚, 罗施中, 赵玉芬

生命有机磷教育部重点实验室, 化学系, 生命科学院, 清华大学, 北京 100084

收稿日期:2001-07-22 出版日期:2001-12-31 发布日期:2001-12-31
通讯作者: 李艳梅(1964年出生),女,博士,教授,博士生导师,从事生物有机化学研究.
基金资助:
国家自然科学基金(批准号:20072023)和高等学校青年教师教学和科研奖励基金资助.

The Fmoc-Strategy Solid-Phase Synthesis of O-Phosphopeptide

CHEN Shui-Bing, LI Yan-Mei, ZHAO Gang, LUO Shi-Zhong, ZHAO Yu-Fen

The Key Laboratory for Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China

Received:2001-07-22 Online:2001-12-31 Published:2001-12-31

摘要/Abstract

摘要：

以Fmoc-策略固相合成方法为基础,以亚磷酰胺为磷酸化试剂,分别以总体磷酸化法和单体磷酸化法合成了多种磷肽、修饰磷肽及其对应的非磷酸化多肽,并以乙腈/水/0.06%三氟乙酸为洗脱体系,用HPLC对磷肽和多肽进行分离.肽链的长度增加,总体法的磷酸化效率降低;这种基于Fmoc-策略的单体磷酸化法目前只适用于含酪氨酸磷肽的合成.

关键词: O-磷酸化多肽, Fmoc-策略固相合成, 总体磷酸化法, 单体磷酸化法

Abstract:

The phosphopeptide and corresponding nonphosphopeptide was synthesized with the Fmoc strategy solid-phase synthesis method. Using N,N-diethyl-dialkyl-phosphoramidity as phosphorylating reagent, some phosphopeptides were obtained by the use of the golble phosphorylation approach, others were obtained from the phosphorylated building block. The peptides were purified with HPLC. The longer the phosphopeptide, the lower the phosphorylating efficiency, Up to now, this Fmoc strategy solid-phase synthesis method with the step-wise phosphotylation approach only can be used in the synthesis of phosphopeptide with Tyr.

Key words: O-phosphopeptide, Fmoc strategy solid-phase synthesis, the golble phosphoiyiation approach, the step-wise phosphotylation approach

中图分类号:

Q516.2

TrendMD:

陈水冰, 李艳梅, 赵刚, 罗施中, 赵玉芬. 基于Fmoc策略的O-磷酸化多肽化学合成研究. 高等学校化学学报, 2001, 22(S1): 106.

CHEN Shui-Bing, LI Yan-Mei, ZHAO Gang, LUO Shi-Zhong, ZHAO Yu-Fen. The Fmoc-Strategy Solid-Phase Synthesis of O-Phosphopeptide. Chem. J. Chinese Universities, 2001, 22(S1): 106.