高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (7): 1114.

• 研究快报 • 上一篇    下一篇

使用一维机械模板的高分辨阿达玛变换显微图像探测技术研究

陈观铨, 唐宏武, 周锦松, 吴琼水   

  1. 武汉大学化学与分子科学学院测试中心, 武汉 430072
  • 收稿日期:2001-02-08 出版日期:2001-07-24 发布日期:2001-07-24
  • 通讯作者: 唐宏武(1969年出生),男,博士,副教授,主要从事分子光谱分析和光谱仪器研究.
  • 基金资助:

    国家自然科学基金(批准号:20075019);国家科技部“九五”科学仪器攻关项目(批准号:96-A23-C-1-008);武汉市青年科技“晨光计划”项目(批准号:995004078G)

Studies on High-resolution Hadamard Transform Microscopic Image Detection System Using One-dimensional Mechanical Mask

CHEN Guan-Quan, TANG Hong-Wu, ZHOU Jin-Song, WU Qiong-Shui   

  1. Centre of Analysis and Measurements, School of Chemical and Molecular Science, Wuhan University, Wuhan 430072, China
  • Received:2001-02-08 Online:2001-07-24 Published:2001-07-24

PDF (PC)

被引次数

12

关键词: 阿达玛变换, 图像分析, 荧光显微术

Abstract: Anovel Hadamard transform(HT) microscopic image detection system which can generate 511× 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexing is performed with a 511-slit one-dimensional Hadamard mask and a highly sensitive linear CCD. The weak native fluorescence imaging for Zephyranthes candida (Lindl.) Herb. Pollen cells can be realized within 1 min. The spatial resolution of 06 μm per pixel has been achieved with a 40× objective. The imaging speed, sensitivity and spatial resolution are satisfactory, hence the system has a good application prospect in cell biology and medicine. Some factors that influence imaging speed and quality have been discussed preliminarily.

Key words: Hadamard transform, Image analysis, Fluorescence microscopy

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