高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (6): 922.

• 研究简报 • 上一篇    下一篇

水介质钯卟啉室温磷光探针与小牛胸腺DNA作用的光谱特性

袁雯1, 晋卫军2, 董川2   

  1. 1. 山西大学师范学院化学系, 太原 030012;
    2. 山西大学化学系, 太原 030006
  • 收稿日期:2000-04-27 出版日期:2001-06-24 发布日期:2001-06-24
  • 通讯作者: 袁　雯(1966出生),女,硕士,副教授,主要从事分子光谱分析研究.
  • 基金资助:

    国家自然科学基金(批准号:29875016);山西省教育委员会科技开发项目(批准号:99039);山西省自然科学基金(批准号:991

Spectroscopic Characteristics of RTP Palladium-porphyrin Probe Interacting with DNA in Aqueous Solution

YUAN Wen1, JIN Wei-Jun2, DONG Chuan2   

  1. 1. Department of Chemistry, Normal College, Shanxi University, Taiyuan 030012, China;
    2. Department of Chemistry, Shanxi University, Taiyuan 030006, China
  • Received:2000-04-27 Online:2001-06-24 Published:2001-06-24

PDF (PC)

被引次数

60

关键词: 钯卟啉, 室温磷光探针, 小牛胸腺DNA

Abstract: The characteristics of absorption, fluorescence, room temperature phosphorescence(RTP) spectra of meso tetrakis(4-N-trimethylaminobenzyl)porphin(TAPP) and Palladium porphyrin(Pd-TAPP) probe in the presence and absence of ct-DNAhas been studied. Pd-TAPP shows a very weak RTPemission in aqueous solution and strong RTP emission in the presence of DNA. Maximum RTPwas observed at pH=7, with maximum excitation and emission wavelengths at 414 nm and 684 nm, respectively. RTPdecay follows a first order exponential equation, RTPlifetime (τ) of probe is (0.72±0.03)ms. The detection limit of ct-DNA may reach 8.8×10-7 mol/L. The relative standard deviation(RSD) is less than ±6.6% in the linear range.

Key words: Palladium-porphyrin, Room temperature phosphorescence probe, ct-DNA

中图分类号: 

TrendMD: