高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (6): 908.

• 研究论文 • 上一篇    下一篇

急/慢性粒细胞白血病患者骨髓血细胞的FTIR研究

高体玉1, 慈云祥1, 付爽2, 王德炳2   

  1. 1. 北京大学化学系, 北京 100871;
    2. 北京大学医学院血液病研究所, 北京 100044
  • 收稿日期:2000-06-22 出版日期:2001-06-24 发布日期:2001-06-24
  • 通讯作者: 慈云祥(1926年出生),男,教授,博士生导师,主要从事生物化学分析研究.
  • 基金资助:

    国家自然科学基金(批准号:39730160);中国博士后科学研究基金资助.

Fourier Transform Infrared Spectroscopic Investigation of Human Blood Cells in Bone Marrow from AML and CML Patients

GAO Ti-Yu1, CI Yun-Xiang1, FU Shuang2, WANG De-Bing2   

  1. 1. Department of Chemistry, Peking University, Beijing 100871, China;
    2. Institute of Hematology, Medical College, Peking University, Beijing 100044, China
  • Received:2000-06-22 Online:2001-06-24 Published:2001-06-24

摘要: 研究了急性粒细胞白血病(AML)和慢性粒细胞白血病(CML)患者的骨髓淋巴细胞、粒细胞和红细胞的傅立叶红外光谱(FTIR).结果发现:(1)这三种细胞FTIR存在着差异性.(2)AML与CML之间淋巴细胞和粒细胞的谱图差异性不明显,但红细胞的谱图却存在可识别的差异性.与CML红细胞相比,AML红细胞酰胺带较宽,AML红细胞蛋白质二级结构中α-螺旋结构的含量较低;1540cm-1与1084cm-1吸收峰面积的比值较低,范围较窄.该结果提示,AML和CML骨髓血红细胞中核红细胞的数量分布不同,同时提示骨髓红细胞的成熟可能与白血病细胞的增殖有关.

关键词: FTIR, 白血病, 骨髓血细胞

Abstract: Eight samples of bone marrow coming from patients affected by acute myeloid leukemia (AML) and nine samples from patients suffered from chronic myeloid leukemia(CML) were studied by FTIRspectroscopy. Obvious differences are observed among the erythrocytes, granulocytes and lymphocytes. No significant differences are found in the granulocytes/lymphocytes between AMLand CMLsamples whereas the spectroscopic differences of leukemic erythrocytes are observed. The ratios of the integrated areas of the band at 1540 cm-1 and 1084 cm-1 in the spectra of erythrocytes from AMLare closer and lower than the corresponding ratios from CMLsamples. Compared to that of CML, the erythrocytes from AMLshow broader amide band Ⅰ and have a higher percentage of α helix in the secondary structures of proteins. These differences indicate the different contributions of red cells in erythrocytic series in the erythropoietic development from proerythroblast to the matured erythrocytes. The results imply the maturation of red cells in bone marrow is related to the proliferation of leukemic cells. The noticeable differences may have important implications for analyzing and evaluating leukemia.

Key words: Fourier transform infrared spectroscopy, Leukemia, Blood cells in bone marrow

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