高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (4): 563.

• 研究简报 • 上一篇    下一篇

高效液相色谱法分离/蒸发光散射和紫外检测法测定天麻中天麻甙含量

魏泱, 丁明玉, 李红霞   

  1. 清华大学化学系, 北京 100084
  • 收稿日期:2000-01-26 出版日期:2001-04-24 发布日期:2001-04-24
  • 通讯作者: 丁明玉(1960年出生),男,博士,副教授,从事色谱与中药有效成分研究.

Determination of Gastrodin in Gastrodia elata Blume by HPLC with ELSD and DAD

WEI Yang, DING Ming-Yu, LI Hong-Xia   

  1. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2000-01-26 Online:2001-04-24 Published:2001-04-24

关键词: 高效液相色谱法, 蒸发光散射检测法, 二极管阵列检测法, 天麻, 天麻甙

Abstract: Anormal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n-hexane/methanol/ethyl acetate(volume ratio 6:3:2 ) as the mobile phase, was established for the direct determination of active constituents-gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume. An silica gel column Zorbax RX-SIL[46 mm (i.d.)×25 cm, 5 μm] was employed. Regression equations revealed the linear relationships( r=0998 and 0999) between the peak areas of GAmeasured by ELSDand DADand its concentration. The recoveries of GAmeasured by ELSDand DADare 104.22% and 97.65%, respectively. The contents of GAmeasured by ELSDand DADare 139 mg/g(2.79% RSD) and 12.1 mg/g(2.99% RSD) respectively in Gastrodia elata Blume. The detection limits of ELSDand DAD( S/N=3) are 3 mg/Land 1 mg/Lrespectively. This method is simple, selective and sensitive.

Key words: High performance liquid chromatography, Evaporative light-scattering detection, Diode array detection, Gastrodia elata Blume, Gastrodin

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