高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (10): 1672.

• 研究论文 • 上一篇    下一篇

侧链键接腺嘌呤的高分子核酸类似物对N-磷酰化氨基酸偶联尿苷反应的模板效应

相红旗1, 胡建军1, 江洋1, 杨成对2, 赵玉芬1, 苏炳禄3, 王光辉3   

  1. 1. 清华大学生命科学与工程院化学系生命有机磷化学教育部重点实验室;
    2. 清华大学分析中心, 北京 100084;
    3. 中国科学院化学研究所, 北京 100080
  • 收稿日期:2000-08-29 出版日期:2001-10-24 发布日期:2001-10-24
  • 通讯作者: 赵玉芬(1948年出生),女,博士,博士生导师,中国科学院院士,主要从事生物有机化学研究.E-mail:tp2dch@mail.tsinghua.edu.cn E-mail:tp-dch@mail.tsinghua.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:39870415)资助

Template Effect of Adenine-contained Polymer Nucleic Acid Analog on the Coupled Action of Uridine by N-(O,O’-Diisopropyl)phosphoryl Alanine

XIANG Hong-Qi1, HU Jian-Jun1, JIANG Yang1, YANG Cheng-Dui2, ZHAO Yu-Fen1   

  1. 1. Key Laboratoryf or Bioorganic Phosphorus Chemistryof Educational Ministry;
    2. Analysis Center, Depa rtment of Chemistry, Schoolof Life Sciencesand Engineering, Tsinghua University, Beijing 100084, China;
    3. Instituteof Chemistry, Chinese Academy of Sciences, Beijing 100080, China
  • Received:2000-08-29 Online:2001-10-24 Published:2001-10-24

PDF (PC)

被引次数

1

摘要： 将高分子核酸类似物聚甲基丙烯酸-β-(N7-腺嘌呤基)乙酯加入到尿苷与N-(O,O-二异丙基)-磷酰化丙氨酸的反应体系之中,研究其对此反应体系的影响.结果表明,聚甲基丙烯酸-β-(N7-腺嘌呤基)乙酯参与了尿苷与磷酰化丙氨酸间的相互作用,并促进了具有较高离子密度、较大的流体动力学体积的大分子量产物及UpU,UpUpU等寡核苷酸片断的生成,表现出明显的模板效应.不含腺嘌呤的对应高分子则无此模板效应,聚甲基丙烯酸-β-(N7-腺嘌呤基)乙酯对尿苷与N-(O,O-二异丙基)-磷酰化丙氨酸反应的模板效应并不是由于其高分子主链引起的,而应归因于其侧链所挂的腺嘌呤碱基的存在,此效应对不同的核苷具有一定的特异性.

关键词: 磷酰化氨基酸, 核苷, 高分子核酸类似物, 模板效应

Abstract: The effect of poly[ β-(N7 adeninyl)ethyl methacrylate] on the reaction of uridine with N-( O,O'-diisopropyl)phosphoryl alanine was studied. It was found that the adenine-contained polymer nucleic acid analog acted as a template to accelerate the coupled action of uridine by phosphoryl alanine. In the presence of the nucleic acid analog, the yield of uridyl nucleotides and uridyl oligonucleotides were increased significantly. The template effect of poly[β-(N7-adeninyl)ethyl methacrylate] showed a selectivity to the coupled action of uridine but not the adenosine. In addition, since the skeleton polymethacrylate did not show any catalytic effect, the template effect of poly[ β-(N7-adeninyl)ethyl methacrylate] must the due to the adenine-bonded side chain.

Key words: Phosphoryl amino acid, Nucleoside, Polymer nucleic acid analog, Template effect

中图分类号: 

TrendMD: