高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (12): 1864.

• 研究简报 • 上一篇    下一篇

丝组二肽所含基团在其切割DNA反应中的作用

万荣, 王宁, 赵刚, 赵玉芬   

  1. 清华大学生命科学与工程研究院, 教育部生命有机膦化学开放实验室, 北京 100084
  • 收稿日期:2000-01-14 出版日期:2000-12-24 发布日期:2000-12-24
  • 通讯作者: 赵玉芬(1948年出生),女,博士,教授,博士生导师,中国科学院院士,主要从事生命有机磷化学的研究.
  • 基金资助:

    国家自然科学基金(批准号:29632004)资助

Seryl-histidine Dipeptide Side Chains Effects on Its DNA Cleavage Reaction

WAN Rong, WANG Ning, ZHAO Gang, ZHAO Yu-Fen    

  1. School of Life Science and Engineering, Bioorganic Phosphorus Chemistry Laboratory of Educational Ministry, Tsinghua University, Beijing 100084, China
  • Received:2000-01-14 Online:2000-12-24 Published:2000-12-24

PDF (PC)

被引次数

25

关键词: 丝组二肽, DNA的切割, 氨基酸侧链效应, 切割机理

Abstract: The effects of the amino, hydroxyl, imidazole and carboxyl groups of Ser-His on DNAcleavage reaction were studied.It was found that the amino and hydroxyl groups were essential for the DNAcleavage activity of Ser-His, and that anyone of them blocked would result into the loss of cleavage activity.The carboxyl group was not the necessary groupThe presence of the imidazole group would enhance the cleavage activity.However, the histidyl-serine dipeptide, as the formula isomer of Ser-His with the inverse peptide sequence, could not cleave DNAat all.Based on the above experimental results, a presumed cleavage mechanism of Ser-His on DNAwas proposed

Key words: Seryl-histidine dipeptide, Cleavage on DNA, Amino acids side chains effects, Cleavage mechanism

中图分类号: 

TrendMD: