高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (12): 1832.

• 论文 • 上一篇    下一篇

一种新型毛细管区带电泳谱图记录模式的研究

张红医1,2,3, 李前锋1, 刘惠涛1, 陈兴国1, 胡之德2   

  1. 1. 兰州大学化学化工学院, 兰州 730000;
    2. 兰州大学生命科学学院, 兰州 730000;
    3. 河北大学化学系, 保定 071002
  • 收稿日期:1999-11-29 出版日期:2000-12-24 发布日期:2000-12-24
  • 通讯作者: 胡之德(1931年出生),男,教授,博士生导师,主要从事毛细管电泳、微波-FIA和人工神经网络在分析化学中的应用等研究.
  • 基金资助:

    中国博士后科学基金资助

The Investigation of a New Recording Mode for Capillary Zone Electrophoresis

ZHANG Hong-Yi1,2,3, LI Qian-Feng1, LIU Hui-Tao1, CHEN Xing-Guo-HU Zhi-De1, WANG Rui2   

  1. 1. Collegeof Chemistryand Chemical Engineering, Lanzhou University, Lanzhou 730000, China;
    2. Schoolof Life Sciences, Lanzhou University, Lanzhou 730000, China;
    3. Departmentof Chemistry, Hebei University, Baoding 071002, China
  • Received:1999-11-29 Online:2000-12-24 Published:2000-12-24

PDF (PC)

被引次数

12

摘要： 提出一种以电通量域模式(吸收信号对电通量作图)代替以时间域模式(吸收信号对时间作图)记录毛细管区带电泳谱图的新模式.在阐述了这种记录模式优点的基础上,用常用的硼砂和磷酸盐体系在不同的电压和固定电压下分离了4种药物,其迁移时间的相对标准偏差分别在41.5%(不同电压下)和1.90%以上(固定电压),而相应的电通量的标准偏差都在1.05%以下,这进一步从实验角度证实了电通量域模式记录电泳谱图的优点:具有极强的抗电场强度波动和温度波动的能力,提高了再现性.以电通量模式记录电泳谱图,还可在一定程度上提高峰面积的重现性.这种记录模式降低了对仪器本身的要求(高压电压电源的稳定性和恒温装置),有可能促进国产低价位毛细管电泳仪的广泛使用

关键词: 毛细管区带电泳, 电通量, 迁移时间, 峰面积

Abstract: Since capillary zone electrophoresis emerged as a powerful separation tool, it has been a common practice to record the electropherogram in terms of the time domain.In this recording mode, the migration time often has a considerable change as the potential gradient and temperature inside the capillary do not remain constant in different runs.In this paper, attempt is made to solve the problem in a new way.The electric flux, defined as the amount of electric charge following a cross section of the capillary in a time interval, takes the place of the time as the domain in an electropherogram.At first, it is theoretically demonstrated that as regards to the reproducibility of separation result the use of the electric flux domain is superior to that of the time domain.Then the performances of electric flux domain and time domain are compared in two typical background electrolyte systems, 20 mmol/L Na2B4O7(pH=9.18) and 10mmol/Lphosphate (pH=8.28).When 10mmol/Lphosphate is used as the background electrolyte and applied voltage varies from10to25 kV(the corresponding temperatures at different voltages are also calculated from our method.), the reproducibility in migration time for berberine hydrochloride, antipyrine, sulfadimidine and sulfadiazine is41.7%, 41.8%, 41.5% and42.2%, respectively.In contrast, the reproducibility in electric flux for the above analytes is1.05%, 0.89%, 0.55% and0.84%, respectively.The reproducibilities in peak areas obtained from the two recording modes under the same experimental conditions are also compared.The relative standard deviation in area obtained from time mode for berberine hydrochloride, antipyrine, sulfadimidine and sulfadiazine is 42.5%, 44.5%, 42.9%, 45.0%, respectively.In contrast, the reproducibility in electric flux for the above analytes is3.79%, 2.83%, 3.02% and5.19%, respectively.Besides, the relative standard deviations in migration time and electric flux are compared in 12runs made consecutively at a fixed voltage.The relative standard deviation in electric flux (<0.7%) is lower than that in migration time (>1.8%)

Key words: Capillary zone electrophoresis, Electric flux, Migration time, Peak area

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