高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (11): 1631.

• 研究快报 • 上一篇    下一篇

细胞色素b5突变体(E44A/E48A/E56A/D60A)的溶液结构研究

吴义兵1,3, 陆军1,3, 钱程明1, 李二成2, 王金凤2, 唐雯霞1, 朱德熙3, 王韵华4, 王文虎4, 陆军霞4, 谢毅4, 黄仲贤4   

  1. 1. 南京大学配位化学国家重点实验室, 南京 210093;
    2. 中国科学院生物大分子国家重点实验室, 北京 100101;
    3. 南京大学生化药物国家重点实验室, 南京 210093;
    4. 复旦大学化学系, 上海 200433
  • 收稿日期:2000-05-16 出版日期:2000-11-24 发布日期:2000-11-24
  • 通讯作者: 唐雯霞(1934年出生),女,教授,博士生导师,从事配位化学研究; 黄仲贤(1940年出生),男,教授,博士生导师,从事生物无机化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29731030;39870166)资助

The Solution Structure of Cytochrome b5 Mutant(E44A/E48A/E56A/D60A)

WU Yi-Bing1,3, LU Jun1,3, QIAN Cheng-Ming1, LI Er-Cheng2, WANG Jin-Feng2, TANG Wen-Xia1, ZHU De-Xi3, WANG Yun-Hua4, WANG Wen-Hu4, LU Jun-Xia4, XIE Yi4, HUANG Zhong-Xian4   

  1. 1. State Key Laboratoryof Coordination Chemistry, Nanjing University, Nanjing 210093, China;
    2. National Laboratoryof Biomacromolecules, Academic Sinica, Beijing 100101, China;
    3. State Key Laboratoryof Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China;
    4. Departmentof Chemistry, Fudan University, Shanghai 200433, China
  • Received:2000-05-16 Online:2000-11-24 Published:2000-11-24

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关键词: 细胞色素b5, 突变体, NMR, 溶液结构

Abstract: The solution structure of bovine cytochrome b5 mutant (E44A/E48A/E56A/D60A) has been obtained from 1H NMR spectra measured at 600 MHz. The final RMSD values with respect to the average structure are (0.056±0.009) nm and (0.105±0.010) nm for backbone and all heavy atoms, respectively. The solution structure, when compared with the X-ray structure of wild type, has no significant changes in the whole folding and secondary structure. Small changes are restricted to local conformation near mutated sites.

Key words: Cytochrome b5, Mutant, NMR, Solution structure

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