高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (1): 62.

• 论文 • 上一篇    下一篇

利用聚合脂质体的沉淀可逆性固定STI及其PL-STI对胰蛋白酶的亲和作用

张文涛1, 于凯2, 孙彦2   

  1. 1. 内蒙古民族师范学院化学系, 通辽 028043;
    2. 天津大学化工系, 天津 300072
  • 收稿日期:1998-12-31 出版日期:2000-01-24 发布日期:2000-01-24
  • 通讯作者: 于　凯(1963年出生),男,博士,副教授,主要从事生物化工研究.
  • 基金资助:

    国家自然科学基金(批准号:29502009)资助

Immobilization of STI on Polymerized Liposome by Inter Arm and Its Affinity Adsorption for Trypsin

ZHANG Wen-Tao1, YU Kai2, SUN Yan2   

  1. 1. Department of Chemistry, Neimenggu Nationality Teachers College, Tongliao 028043, China;
    2. Department of Chemical Engineering, Tianjin University, Tianjin 300072, China
  • Received:1998-12-31 Online:2000-01-24 Published:2000-01-24

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被引次数

1

摘要： 通过双功能试剂戊二醛、二甘醇二酸酐和1,4-丁二醇双缩水甘油醚等将大豆胰蛋白酶抑制剂STI固定到由二十五-10,12-二块-1-醇磷脂乙醇胺形成的聚合脂质体上.同时探讨了其作为亲和沉淀吸附剂对对胰蛋白酶的亲和作用.在PL沉淀过程中,胰蛋白酶有20%～60%从PL-STI上解离下来.PL-STI可作为酶的亲和沉淀吸附剂.

关键词: 聚合脂质体, 胰蛋白酶, 亲和沉淀

Abstract: Soybean trypsin inhibitor(STI) was immobilized on polymerized liposome of pentacose-10,12-diene-1-ol-phospho ethanolamine by diglycolic anydride, glutaraldehyde and 1,4-butanediol diglycidyl ether. In the course of precipitation, 20% to 60% trypsin does cleavage from PL-STI and shows PL-STI can be used as the affinity precipitation adsorbent of enzyme.

Key words: Polymerized liposome, Trypsin, Affinity precipitation

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