摘要：

Loulu is a traditional Chinese medicine which has obvious activities in anti-peroxidantion, anti-atherosclofosis and immunopotentintion. The pharmacological activities of ecdysterone in Loulu are in good agreement with the curative effects of Loulu. Therefore, the quantitative determination of ecdysterone in Loulu will be helpful in further researching the medicinal mechanism. The components of Loulu are very complex. Many overlapping peaks from interference will be present in chromatograms if the sample is directly determined by HPLC. So it is very important to search for a simple, rapid and effective sample pretreatment technique. Flow injection analysis, as an effective sample treatment technique, has applied in many fields. In this work, a flow injection micro-column system for the sample pretreatment coupling HPLC was proposed. Ecdysterone in.Loulu was pre-separated with the designed system, and then determined by HPLC. The separation performance, the influence factors of the pre-separation and the operation parameters of the HPLC have investigated in detail. The experimental results demonstrated that the present system is simple, efficient and no pollution. The method was applied to the determination of ecdysterone in real samples.

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