高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 496.

• Biological Analysis • 上一篇    下一篇

Use of Ternary Ion-association Color Change on Membrane Filter for Determination of Protein

Keitaro Yoshimoto, Emiko Kaneko, Takao Yotsuyanagi   

  1. Department of Applied Chemistry, Graduate School of Engineering, Tohoku University, Sendai, 980-8579, Japan
  • 出版日期:1999-12-31 发布日期:1999-12-31

Use of Ternary Ion-association Color Change on Membrane Filter for Determination of Protein

Keitaro Yoshimoto, Emiko Kaneko, Takao Yotsuyanagi   

  1. Department of Applied Chemistry, Graduate School of Engineering, Tohoku University, Sendai, 980-8579, Japan
  • Online:1999-12-31 Published:1999-12-31

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摘要：

The dye-binding assay has become an important method for protein analysis,primarily due to its convenience and high sensitivity. Only one simple reagent such as triphenylmethan-dye is required, and color development is generally rapid. The aim of this work is to develop a superior visual method with a high sensitivity for proteins in an aqueous solution. Here we show a novel protein assay based on the membrane filtration of the ternary associates and subsequent color change of the chromophore.

Abstract:

The dye-binding assay has become an important method for protein analysis,primarily due to its convenience and high sensitivity. Only one simple reagent such as triphenylmethan-dye is required, and color development is generally rapid. The aim of this work is to develop a superior visual method with a high sensitivity for proteins in an aqueous solution. Here we show a novel protein assay based on the membrane filtration of the ternary associates and subsequent color change of the chromophore.

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