高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 493.

• Biological Analysis • 上一篇    下一篇

Determination of Nucleic Acids by the Columinescence Effect of Tb-Gd-Nucleic Acid System

YANG Jing-He1, WU Xia1, CAO Xi-Hui1, SUN Li-Mei1, LIN Cun-Guo2, ZHANG Gui-Ling3, HAN Rong-Jiang4   

  1. 1. Department of Chemistry, Sliandong University, Jinan 250100, P. R. China;
    2. Branch of LUOYANG Ship Material Research Institute, Qingdao 266071, P. R. China;
    3. College of Chemistry and Chemical Engineering, Qingdao Ocean University, 266003, P. R. China;
    4. Department of light Engineering, Shandong Institute of light Industry, Jinan 250100, P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31

Determination of Nucleic Acids by the Columinescence Effect of Tb-Gd-Nucleic Acid System

YANG Jing-He1, WU Xia1, CAO Xi-Hui1, SUN Li-Mei1, LIN Cun-Guo2, ZHANG Gui-Ling3, HAN Rong-Jiang4   

  1. 1. Department of Chemistry, Sliandong University, Jinan 250100, P. R. China;
    2. Branch of LUOYANG Ship Material Research Institute, Qingdao 266071, P. R. China;
    3. College of Chemistry and Chemical Engineering, Qingdao Ocean University, 266003, P. R. China;
    4. Department of light Engineering, Shandong Institute of light Industry, Jinan 250100, P. R. China
  • Online:1999-12-31 Published:1999-12-31

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摘要：

Because rare earth ions have some characteristics such as narrow spectral width,long fluorescence life, large stokes shift and strongly binding with biological molecules, therefore they are usually as fluorescence probes to study nucleic acids, However the sensitivity is low. It is found that the fluorescence of Tb-nucleic acids (fish sperm DNA and yeast RNA) can increased by Sc3+,Y3+,La3+, Gd3+ and Lu3+, among which Gd3+ and Lu3+ have the greatest enhancement. This is a newly found co-luminescence effect.

Abstract:

Because rare earth ions have some characteristics such as narrow spectral width,long fluorescence life, large stokes shift and strongly binding with biological molecules, therefore they are usually as fluorescence probes to study nucleic acids, However the sensitivity is low. It is found that the fluorescence of Tb-nucleic acids (fish sperm DNA and yeast RNA) can increased by Sc3+,Y3+,La3+, Gd3+ and Lu3+, among which Gd3+ and Lu3+ have the greatest enhancement. This is a newly found co-luminescence effect.

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